T-Helper Cells

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106,99 

Methods and Protocols, Methods in Molecular Biology 1193

ISBN: 1493912119
ISBN 13: 9781493912117
Herausgeber: Ari Waisman/Burkhard Becher
Verlag: Humana Press
Umfang: xi, 229 S., 14 s/w Illustr., 24 farbige Illustr., 229 p. 38 illus., 24 illus. in color.
Erscheinungsdatum: 23.08.2014
Auflage: 1/2014
Produktform: Gebunden/Hardback
Einband: Gebunden

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo, and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Artikelnummer: 6647173 Kategorie:

Beschreibung

Autorenporträt

InhaltsangabePart I. Isolation of T Cells from the Tissues 1. Isolation of T Cells from the Skin Beate Lorenz and Esther von Stebut 2. Isolation of Leukocytes from Mouse Central Nervous System Iva Lelios and Melanie Greter 3. Isolation of T Cells from the Gut Sonja Reißig, Christopher Hackenbruch, and Nadine Hövelmeyer 4. T Cell Isolation from Mouse Kidneys Isis Ludwig-Portugall and Christian Kurts  Part II. Analysis of T Cell Function and Phenotypes 5. Intracellular Staining for Cytokines and Transcription Factors Florian Mair and Vinko Tosevski 6. Surface Capture StainingVinko Tosevski and Florian Mair 7. Mass Cytometry Analysis of Human T Cell Phenotype and Function Evan W. Newell and Lai Li Yun 8. Detection of NF- B Pathway Activation in T Helper Cells Oliver Gorka, Stefan Wanninger, and Jürgen Ruland 9. Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells Christian Thomas Mayer and Tim Sparwasser 10. In vitro Generation of Microbe Specific Human Th17 Cells Julia M. Braun and Christina E. Zielinski 11. In vitro Polarization of T-Helper Cells Julie Rumble and Benjamin Segal  Part III. In vivo Models of T Cell Function 12. A Method for the Generation of TCR Retrogenic Mice Elisa Kieback, Ellen Hilgenberg, and Simon Fillatreau 13. Mouse Models of Allergic Airway Disease Helen Meyer-Martin, Sebastian Reuter, and Christian Taube 14. Induction of Colitis in Mice (T-Cell Transfer Model) Benno Weigmann 15. Manipulation of T Cell Function Conditional Gene Targeting in T Cells  Anna Sledzinska, Lynsey Fairbairn, and Thorsten Buch 16. Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate LymphocytesC.T. Wohn, S. Pantelyushin, J.L. Ober-Blöbaum, and B.E. Clausen 17. Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells Rhoanne C. McPherson, Helen E. Cambrook, Richard A. O'Connor, and  Stephen M. Anderton 18. Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease George X. Song-Zhao and Kevin J. Maloy 19. Analysis of Chromosomal Aberrations in Murine HCC Kristian Unger and Mathias Heikenwälder

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