Beschreibung
Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.
Inhaltsverzeichnis
Part I: Introduction 1. Regulatory T Cells: History and Perspective Shimon Sakaguchi Part II: In vitro 2. In vitro Treg Suppression Assays Lauren W. Collison and Dario A.A. Vignali 3. Generation of T Cell Hybridomas from Naturally-Occurring FoxP3+ Regulatory T Cells Nagendra Singh, Rafal Pacholczyk, Makio Iwashima, and Leszek Ignatowicz 4. In vitro and In vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells Kim J. Hasenkrug and Lara M. Myers 5. Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus Donald M. Simons and Andrew J. Caton 6. ChIP-on-Chip for FoxP3 Ye Zheng 7. Live Imaging of Dendritic Cell-Treg Cell Interactions Milka Sarris and Alexander G. Betz Part III: In vivo 8. Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In vivo Nadia M. Jeremiah and Adrian Liston 9. In vivo Treg Suppression Assays Creg J. Workman, Lauren W. Collison, Maria Bettini, Meenu R. Pillai, Jerold E. Rehg, and Dario A.A. Vignali 10. In vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model Katharina Lahl and Tim Sparwasser 11. Antigen-Specific Induction of Regulatory T Cells In vivo and In vitro Carolin Daniel, Hidde Ploegh, and Harald von Boehmer 12. In vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection Clemence Nouze, Lise Pasquet, and Joost P.M. van Meerwijk Part IV: Human 13. Analysis of Human FoxP3+ Treg Cells Phenotype and Function Eva d¿Hennezel and Ciriaco A. Piccirillo 14. Depletion of Human Regulatory T Cells Amy C. Hobeika, Michael A. Morse, Takuya Osada, Sharon Peplinski, H. Kim Lyerly, and Timothy M. Clay 15. Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In vitro Assay Anya Schneider and Jane H. Buckner 16. Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose Milica Vukmanovic-Stejic, Yan Zhang, Arne N. Akbar, and Derek C. Macallan 17. Flow Cytometric Detection of Human Regulatory T Cells Barbara Fazekas de St. Groth, Erhua Zhu, Suzanne Asad, and Loretta Lee
