Beschreibung
Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biologya''¢ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.
Inhaltsverzeichnis
DNA Damage Detection In Situ, Ex Vivo and In Vivo: Methods and Protocols Vladimir V. Didenko, Editor Table of Contents ________________________________________________________________ Table of Contents Preface Contributors Part 1. DETECTION IN TISSUE SECTIONS 1In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques. Deryk T. Loo 2Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage. Alexander E. Kalyuzhny 3EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level. Antonio Migheli 4In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase. Vladimir V. Didenko 5In Situ Ligation: a Decade and a Half of Experience. Peter J. Hornsby and Vladimir V. Didenko 6In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections. Vladimir V. Didenko 75¿OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach. Vladimir V. Didenko Part 2. DETECTION IN CELL CULTURES 8Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry. Zbigniew Darzynkiewicz and Hong Zhao 9Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis. Zbigniew Darzynkiewicz, Piotr Pozarowski, Brian W. Lee and Gary L. Johnson 10Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Sergey Shaposhnikov, Preben D. Thomsen, and Andrew R. Collins 11Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH). Jose Luis Fernandez, Dioleyda Cajigal, Jaime Gosalvez 12Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells. Jennifer Guerrero-Santoro, Arthur S. Levine, and Vesna Rapic-Otrin Part 3. DETECTION IN LIVE TISSUES, BLOOD, URINE, SPERM 13Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. Roxana M. Vlad, Michael C. Kolios and Gregory J. Czarnota 14Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry. Jagadeesan Nair, Urmila J. Nair, Xin Sun, Ying Wang, Khelifa Arab, and Helmut Bartsch 15ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells. Jodi Hagen, J.P. Houchins and Alexander E. Kalyuzhny 16Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes. Philip Thomas and Michael Fenech 17Buccal Micronucleus Cytome Assay. Philip Thomas and Michael Fenech 18i§-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin. Christophe E. Redon, Asako Nakamura, Olivier Sordet, Jennifer S. Dickey, Ksenia Gouliaeva, Brian Tabb, Scott Lawrence, Robert J. Kinders, William M. Bonner, Olga A. Sedelnikova 19Immunologic Detection of Benzo(a)pyrene-DNA Adducts. Regina M. Santella and Yu-Jing Zhang 20Non-Invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2¿-deoxyguanosine.Vilas Mistry, Friederike Teichert, Jatinderpal.K.Sandhu, Rajinder Singh, Mark D. Evans, , Peter B. Farmer, and Marcus S. Cooke 21Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test. Jose Luis Fernandez, Dioleyda Cajigal, Carmen Lopez-Fernandez, Jaime Gosalvez
