Beschreibung
Influenza virus is an important human pathogen, frequently causing widespread disease and a significant loss of life. Much has been learned about the structure of the virus, its genetic variation, its mode of gene expression and replication, and its interaction with the host immu nologic system. This knowledge has the potential of leading to ap proaches for the control of influenza virus. In addition, research on influ enza virus has led to important advances in eukaryotic molecular and cellular biology and in immunology. A major focus of this book is the molecular biology of influenza virus. The first chapter, which serves as an introduction, describes the structure of each of the genomic RNA segments and their encoded pro teins. The second chapter discusses the molecular mechanisms involved in the expression and replication of the viral genome. In addition to other subjects, this chapter deals with one of the most distinctive features of influenza virus, namely the unique mechanism whereby viral messenger RNA synthesis is initiated by primers deaved from newly synthesized host-cell RNAs in the nudeus. Among the most significant accomplish ments in influenza virus research has been the delineation of the three dimensional structure of the two surface glycoproteins of the virus, the hemagglutinin and neuraminidase. This has provided a structural basis for mapping both the antigenic sites and the regions involved in the major biological functions of these two molecules.
Autorenporträt
Inhaltsangabe1 Genes and Proteins of the Influenza Viruses.- I. Introduction.- A. Structure of the Genome.- II. RNA Segments 1, 2, and 3: The Three Polymerase-Associated Proteins Form a Complex to Transcribe RNA.- A. Three P Proteins.- B. PB1, PB2, and PA Gene Sequences.- C. Functions of the PB1, PB2, and PA Proteins.- D. P Proteins Form a Complex That Migrates to the Cell Nucleus.- E. Influenza B Virus P Proteins and RNA Segments.- F. Influenza C Viruses.- III. RNA Segment 4: Synthesis, Structure, and Function of the Hemagglutinin.- A. General Introduction to the Hemagglutinin.- B. RNA Segment 4 Gene Structure.- C. Three-Dimensional Structure of the Hemagglutinin.- D. Synthesis of HA, Co-translational and Post-translational Modifications, Oligomerization, and the Exocytotic Pathway.- E. Cleavage Activation.- F. Viral Entry and Membrane Fusion.- G. Influenza B Virus Hemagglutinin.- H. Influenza C Virus Glycoprotein.- IV. RNA Segment 5: The Nucleocapsid Protein Forms the Structural Monomer Unit of the Ribonucleoprotein Particles.- A. Structure of RNPs and Involvement in RNA Synthesis.- B. Properties of the Nucleocapsid Protein.- C. Nuclear Transport and Karyophilic Sequences.- D. RNA-Protein Interactions.- E. NP Is a Major Target for Cytotoxic T Lymphocytes.- F. Influenza B Virus NP Gene and Protein.- G. Influenza C Virus NP Gene and Protein.- V. RNA Segment 6: The Neuraminidase.- A. Neuraminidase Function.- B. Structure of Neuraminidase.- C. General Deductions from the Gene Sequence of Influenza A Virus NA.- D. Influenza A Virus Strain Similarities and Differences.- E. N-Terminal Signal Anchor.- F. Three-Dimensional Structure of NA.- G. Influenza B Virus Neuraminidase.- H. Influenza B Virus RNA Segment 6.- I. NB Glycoprotein of Influenza B Virus.- J. Effect of Mutations and Deletions in the Bicistronic mRNA for NB and NA.- K. Influenza C Virus.- VI. RNA Segment 7 of Influenza A Virus: Structure and Synthesis of the Membrane Protein (M1) and an Integral Membrane Protein (M2) from Unspliced and Spliced mRNAs.- A. RNA Segment 7 and Its mRNAs.- B. Membrane Protein (M1).- C. Influenza Virus M2 Protein Structure and Subcellular Localization.- D. Expression of M2 in Eukaryotic Cells from Cloned cDNA.- E. Characterization of a Monoclonal Antibody to the M2 Extracellular Domain.- F. Initial Interaction of M2 with Membranes.- G. Is M2 a Structural Component of Virions?.- H. Function of M2 in Infected Cells.- I. Effect of Amantadine Hydrochloride on M2.- J. Evolution of the Nucleotide Sequence of RNA Segment 7 and the Amino Acid Sequences of M1 and M2.- K. RNA Segment 7 of Influenza B Virus.- L. Membrane Protein of Influenza C Virus.- VII. Influenza A Virus RNA Segment 8: Unspliced and Spliced mRNAs Encode Nonstructural Proteins NS1 and NS2.- A. RNA Segment 8 and Its mRNAs.- B. NS1 Protein.- C. NS2 Protein.- D. RNA Segment 8 of Influenza B Virus: Unspliced and Spliced mRNAs Code for NS1 and NS2.- E. Influenza C Virus RNA Segment 7: Unspliced and Spliced mRNAs Code for NS1 and NS2.- References.- 2 Expression and Replication of the Influenza Virus Genome.- I. Introduction.- II. Viral mRNA Synthesis.- III. Template RNA Synthesis.- IV. Virion RNA Synthesis.- V. Regulation of Viral Gene Expression in Infected Cells.- VI. Interferon-Induced Mx Protein, a Specific Inhibitor of Influenza Virus Replication.- VII. Regulated Splicing of the Viral NS1 and M1 mRNAs.- VIII. Mechanisms for the Selective and Efficient Translation of Influenza Virus mRNAs.- References.- 3 Structure, Function, and Antigenicity of the Hemagglutinin of Influenza Virus.- I. Introduction.- II. Structure of Hemagglutinin.- III. Antigenic Variation.- A. Antigenic Shift.- B. Antigenic Drift.- C. The Importance of Receptor Specificity in Antigenic Change.- IV. Receptor Binding.- A. Structure of the Receptor-Binding Site.- B. The Binding Site of a Receptor-Binding Mutant.- V. Fusion Properties of Hemagglutinin.- A. Acid-Induced Conformational Change of Hemagglutinin.- VI. Conclusions.- Referen
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Springer Verlag GmbH
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E-Mail: juergen.hartmann@springer.com
