Avian Influenza Virus

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Methods in Molecular Biology 436

ISBN: 1588299392
ISBN 13: 9781588299390
Herausgeber: Erica Spackman
Verlag: Humana Press
Umfang: x, 142 S.
Erscheinungsdatum: 28.02.2008
Auflage: 1/2008
Produktform: Gebunden/Hardback
Einband: GEB

With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. Exciting, cutting-edge new methods focus on studying the virus itself and work with avian hosts, an area greatly lacking in research. Following the format of the highly successful Methods in Molecular Biology series, each chapter provides readily reproducible laboratory protocols offering step-by-step instruction and lists of the necessary equipment for the task. Comprehensive and timely, Avian Influenza Virus equips diagnosticians and researchers with the current tools and information they need to learn more about this high impact disease.

Artikelnummer: 992079 Kategorie:

Beschreibung

InhaltsangabeAvian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling MaryLea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. TypeA Influenza Virus Detection and Quantitation by Realtime RTPCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus MaryLea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary PantinJackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus ChangWon Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut

Inhaltsverzeichnis

Avian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut

Autorenporträt

InhaltsangabeAvian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut

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