Beschreibung
The second edition covers a wide range of protein-protein interaction detection topics. Protein-Protein Interactions: Methods and Applications focuses on core technological platforms used to study protein-protein interactions and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing of protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces.Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery.
Autorenporträt
InhaltsangabePART I OVERVIEWS 1 Structural basis of protein-protein interactionsRobert Liddington2 Quantitative analysis of protein-protein interactions Ziad Eletr and Keith Wilkinson3 Protein-protein interaction databases Damian Szklarczyk and Lars Juhl Jensen 4 Computational prediction of protein-protein interactions Tobias Ehrenberger, Lewis C. Cantley, and Michael B. Yaffe5 Structure-based computational approaches for small-molecule modulation of protein-protein interactionsDavid Xu, Bo Wang, and Samy O. Meroueh6 Targeting protein-protein interactions for drug discovery David C. Fry PART II LABEL-FREE BIOSENSORS AND TECHNIQUES 7 Studying protein-protein interactions using surface plasmon resonanceZenata Nikolovska-Coleska8 Resonant waveguide grating for monitoring bimolecular interactions Meng Wu and Min Li 9 Quartz microbalance technology for probing biomolecular interactions Gabriella T. Heller, Alison R. Mercer-Smith, and Malkiat S. Johal10 Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry Sriram Kumaraswamy and Renee Tobias11 Characterization of protein-protein interactions by isothermal titration calorimetryAdrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freiere12 Sedimentation equilibrium studies Ian A. Taylor, John F. Eccleston, and Katrin Rittinger13 Detecting protein-protein interactions by gel filtration chromatographyYan Bai14 Using light scattering to determine the stoichiometry of protein complexes Jeremy Mogridge15 Circular dichroism (CD) analyses of protein-protein interactions Norma Greenfield16 Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy Peter M. Thompson, Moriah R. Beck, and Sharon L. Campbell17 Quantitative protein analysis by mass spectrometry Vishwajeeth Pagala, Anthony High, Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra, Kanisha Kavdia, Yanji Xu, Zhiping Wu, and Junmin Peng18 Using peptide arrays created by the SPOT method for defining protein-protein interactionsYun Young Yim, Katherine Betke, and Heidi Hamm PART III TAG/AFFINITY-BASED METHODS 19 Fluorescence polarization assay to quantify protein-protein interactions Ronald T. Raines20 Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions Alexa L. Mattheyses and Adam I. Marcus21 Utilizing ELISA to monitor protein-protein interactionsZusen Weng and Qinjian Zhao22 Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions Haris G. Vikis and Kun-Liang Guan23 Hexahistidine (6xHis) fusion-based assays for protein-protein interactions Mary C. Puckett24 Studying protein-protein interactions via blot overlay/Far Western blot Randy A. Hall25 Co-immunoprecipitation from transfected cells Yoshinori Takahashi26 In vivo protein cross-linking Fabrice Agou and Michel Véron<
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