Beschreibung
Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.
Autorenporträt
InhaltsangabePart I. Screening and Design Purification Strategies 1. Protein Purification: An OverviewNikolaos Labrou 2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoEIgnacio Amadeo, Laura Mauro, Eduardo Ortí, and Guillermina Forno 3. High Throughput Process Development: I. Process ChromatographyA. S. Rathore and R. Bhambure 4. High Throughput Process Development: II. Membrane ChromatographyA. S. Rathore and S. Muthukumar 5. Media Selection in Ion Exchange Chromatography in a Single MicroplateCharlotte Cabanne and Xavier Santarelli 6. High Throughput Screening of Dye-Ligands for ChromatographySunil Kumar and Narayan S. Punekar 7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette TipsSunil Chhatre and Daniel G. Bracewell Part II. Low-Resolution Protein Purification Methods 8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline Zhengrong GU 9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from PlantsOscar Aguilar and Marco Rito-Palomares 10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic ProteinsA. S. Rathore and R. Bhambure 11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta 12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and PeriplasmArio de Marco 13. Immunoglobulin Purification by Caprylic AcidVictor Morais, Patricia Berasain, and Hugo Massaldi Part III: High-Resolution Protein Purification Methods 14. Affinity Tags in Protein Purification and Peptide Enrichment: An OverviewAna Sofia Pina, Íris L. Batalha, and Ana Cecília A. Roque 15. Expression and Purification of Large Active GST Fusion EnzymesStefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa 16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion ProteinsSharon Williams, Phil Morton, and Dev Baines 17. Zbasic - A Purification Tag for Selective Ion-Exchange RecoveryMy Hedhammar, Johan Nilvebrant, and Sophia Hober 18. An Orthogonal Fusion Tag for Efficient Protein PurificationJohan Nilvebrant, Mikael Åstrand, and Sophia Hober 19. Phage Display of Engineered Binding ProteinsMark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servé W.M. Kengen, and John van der Oost 20. Biomimetic Affinity Ligands for Protein PurificationIsabel T. Sousa and M. Ângela Taipa 21. Synthesis and Application of Dye-Ligand Affinity AdsorbentsEvangelia Chronopoulou and Nikolaos E. Labrou 22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein PurificationS. A. Camperi, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone 23. Affinity Chromatography of Proteins on Monolithic ColumnsE.G. Vlakh, G.A. Platonova, and T.B. Tennikova 24. Sample Displacement Batch Chromatography of ProteinsMarta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization 25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS ApproachesCatalin E. Doneanu, Weibin Chen, and St John Skilton
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE
E-Mail: juergen.hartmann@springer.com
