Methods in Membrane Lipids

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Methods in Molecular Biology 1232

ISBN: 149391751X
ISBN 13: 9781493917518
Herausgeber: Dylan M Owen
Verlag: Humana Press
Umfang: xi, 327 S., 28 s/w Illustr., 15 farbige Illustr., 327 p. 43 illus., 15 illus. in color.
Erscheinungsdatum: 21.10.2014
Auflage: 2/2014
Produktform: Gebunden/Hardback
Einband: GEB

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico.  Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties, and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative  and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

Artikelnummer: 7018727 Kategorie:

Beschreibung

Inhaltsangabe1. Introduction to Membrane LipidsRichard M. Epand 2. Introduction: Membrane properties (Good) for LifeMarek Cebecauer 3. Introduction To Fluorescence Probing Of Biological MembranesAlexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély 4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)Charles Cranfield, Sonia Carne, Boris Martinac,  and Bruce Cornell 5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian CellsDeborah A. Brown 6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein PartitioningKR Levental and  I Levental 7. Asymmetric Giant Lipid Vesicle FabricationPeichi C. Hu and Noah Malmstadt 8. Cholesterol Depletion using Methyl-b-cyclodextrinSaleemulla Mahammad and Ingela Parmryd 9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture SystemsG. Ekin Atilla-Gokcumen and Ulrike S. Eggert  10. Imaging Membrane Order using Environmentally Sensitive FluorophoresG Ashdown and DM Owen 11. 3D Super-resolution Imaging by Localisation Microscopy Astrid Magenau and Katharina Gaus 12. Electron Microscopy Methods for Studying Plasma MembranesAlison J. Beckett and Ian A. Prior 13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force MicroscopyCharles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas 14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane OrderRichard KP Benninger 15. Scanning Fluorescence Correlation Spectroscopy on BiomembranesEduard Hermann, Jonas Ries, and Ana J. García-Sáez 16. X-ray Diffraction of Lipid Model MembranesArwen I.I. Tyler, Robert V. Law, and John M. Seddon  17. Solid State NMR of Lipid Model MembranesArwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law 18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and ApplicationsMichael Carnell, Alex Macmillan, and  Renee Whan 19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live CellsOttavia Golfetto, Elizabeth Hinde, and Enrico Gratton 20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics SimulationsSemen O. Yesylevskyy and Alexander P. Demchenko 21. Computer Simulations Of Phase Separation In Lipid Bilayers and MonolayersSvetlana Baoukina and D. Peter Tieleman                                                                                                                                                            

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