Cell Cycle Synchronization

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160,49 

Methods and Protocols, Methods in Molecular Biology 761

ISBN: 1617791814
ISBN 13: 9781617791819
Herausgeber: Gaspar Banfalvi
Verlag: Humana Press
Umfang: xii, 295 S.
Erscheinungsdatum: 12.07.2011
Auflage: 1/2011
Produktform: Gebunden/Hardback
Einband: GEB

This Methods in Molecular Biologya“¢ volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

Artikelnummer: 1629852 Kategorie:

Beschreibung

Inhaltsangabe1. Overview of Cell Synchronization            Gaspar Banfalvi 2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation            Gaspar Banfalvi 3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells            Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer 4. Large Scale Mitotic Cell Synchronization            Kalyan Dulla and Anna Santamaria 5. Synchronization of Mammalian Cell Cultures by Serum Deprivation            Thomas J. Langan and Richard C. Chou 6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry            Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka 7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes            Takashi Iwamatsu 8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis            Congjun Li 9. Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle            Jennifer A. Fischer and Salvatore Caradonna 10. Synchronization of HeLa Cells            Hoi Tang Ma and Randy Y.C. Poon 11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth            Gaspar Banfalvi 12. Synchronization of Yeast            Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider 13. Synchronization of Pathogenic Protozoans            Staffan Svärd and Karin Troell 14. Synchronization of In Vitro Maturation in Porcine Oocytes            Tamas Somfai and Yuji Hirao 15. Synchronization of Medicago sativa Cell Suspension Culture            Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth 16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)            Yoel Shufaro  and Benjamin E. Reubinoff 17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation            Kap-Hyoun Ko, Robert Nordon, Tracey A. O'Brien, Geoff Symonds, and Alla Dolnikov 18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients            Fabienne Venet, Caroline Guignant, and Guillaume Monneret 19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish            Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky

Inhaltsverzeichnis

1. Overview of Cell Synchronization             Gaspar Banfalvi   2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation             Gaspar Banfalvi   3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells             Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Metivier, Mohamed Jemaà, Santiago Rello-Varona, and Guido Kroemer   4. Large Scale Mitotic Cell Synchronization             Kalyan Dulla and Anna Santamaria   5. Synchronization of Mammalian Cell Cultures by Serum Deprivation             Thomas J. Langan and Richard C. Chou   6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry             Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka   7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes             Takashi Iwamatsu   8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis             Congjun Li   9. Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle             Jennifer A. Fischer and Salvatore Caradonna   10. Synchronization of HeLa Cells             Hoi Tang Ma and Randy Y.C. Poon   11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth             Gaspar Banfalvi   12. Synchronization of Yeast             Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider   13. Synchronization of Pathogenic Protozoans             Staffan Svärd and Karin Troell   14. Synchronization of In Vitro Maturation in Porcine Oocytes             Tamas Somfai and Yuji Hirao   15. Synchronization of Medicago sativa Cell Suspension Culture             Ferhan Ayaydin, Edit Kotogany, Edit Ábraham, and Gabor V. Horvath   16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)             Yoel Shufaro  and Benjamin E. Reubinoff   17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation             Kap-Hyoun Ko, Robert Nordon, Tracey A. O¿Brien, Geoff Symonds, and Alla Dolnikov   18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients             Fabienne Venet, Caroline Guignant, and Guillaume Monneret   19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish             Attila Csikasz-Nagy, Alida Palmisano, and Judit Zamborszky

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