Beschreibung
Fluorescence spectroscopy and imaging, which exploit native tissue fluorescence (autofluorescence), have emerged as non-invasive real-time tissue diagnostic tools and have been under intensive studies in the past years. However, traditional autofluorescence techniques have suffered from tissue scattering and absorption, and thus are generally not efficient for tissue diagnostics. This book introduces a study using polarized autofluorescence and polarized reflectance techniques to reduce tissue scattering and absorption effects and to reveal endogenous optical signatures of skin cancer. Normal and malignant melanocytic cells can be efficiently discriminated using fluorescence anisotropy of cellular NADH. Enzymatic dermis degradation mimicking tumor invasion can be successfully followed by the fluorescence emission and polarized reflectance of the dermis. These results should thus shed lights on the understanding of the endogenous optical signatures associated with cancer development, and should be a valuable reference for researchers working in the fields of biomedical optics and biophotonics.
Autorenporträt
Ye Yuan, PhD in bioengineering: His doctoral research at The University of Toledo, Ohio focused on polarized fluorescence imaging of human skin for melanoma detection. He is currently a postdoctoral researcher working on optical instrumentation for fluorescence lifetime spectroscopy and imaging at McMaster University in Canada.
