Beschreibung
Leland H. Hartwell Director, Fred Hutchinson Cancer Research Center, Nobel Laureate for Medicine, 2001 Yeast has proved to be the most useful single-celled organism for studying the fundamental aspects of cell biology. Resources are now available for yeast that greatly simplify and empower new investigations, like the presence of strains with each gene deleted, each protein tagged and databases on protein-protein interactions, gene regulation, and subcellular protein location. A powerful combination of genetics, cell biology, and biochemistry employed by thousands of yeast researchers has unraveled the complexities of numerous cellular processes from mitosis to secretion and even uncovered new insights into prion diseases and the role of prions in normal biology. These insights have proven, time and again, to foretell the roles of proteins and pathways in human cells. The collection of articles in this volume explores the use of yeast in pathway analysis and drug discovery. Yeast has, of course, supplied mankind's most ubiquitous drug for thousands of years. In one aspect, the role of yeast in drug discovery is much like the role of yeast in other areas of biology. Yeast offers the power of genetics and a repetoire of resources available in no other organism. Using yeast in the study of drug targets and metabolism can help to make a science of what has been largely an empirical activity. A science of drug discovery would permit rigorous answers to important questions.
Autorenporträt
InhaltsangabeForeword Introduction Chapter 1: From DNA Replication to Genome Instability in Schizosaccharomyces pombe: Pathways to Cancer by Julie M. Bailis and Susan L. Forsburg Chapter 2: Dissecting Layers of Mitotic Regulation Essential for Maintaining Genomic Stability by Jeffiner S. Searle and Yolanda Sanchez Chapter 3: Yeast as a Tool in Cancer Research: Nuclear Trafficking by Anita H. Corbett and Adam C. Berger Chapter 4: Studies of Protein Farnesylation in Yeast by Nitika Thaper and Fuyuhiko Tamanoi Chapter 5: From Bread to Bedside: What Budding Yeast has Taught us about the Immortalization of Cancer Cells by Soma S.R. Banik and Christopher M. Counter Chapter 6: Hsp90 Co-chaperones in Saccharomyces Cerevisiae by Marija Tesiccc and Richard F. Gaber Chapter 7: Yeast as a Model System for Studying Cell Cycle Checkpoints by Carmela Palermo and Nancy C. Walworth Chapter 8: Metabolism and Function of Sphingolipids in Saccharomyces Cerevisiae: Relevance to Cancer Research by L. Ashley Cowart, Yusuf A. Hannun, and Lina M. Obeid Chapter 9: Exploring and Restoring the p53 Pathway Using the p53 Dissociator Assay in Yeast by Rainer K. Brachmann Chapter 10: Functional Analysis of the Human p53 Tumor Suppressor and its Mutants using Yeast by Alberto Inga, Francesca Storici and Michael A. Resnick Chapter 11: ABC Transporters in Yeast - Drug Resistance and Stress Response in a Nutshell by Karl Kuchler and Christoph Schüller Chapter 12: The FHCRC/NCI Yeast Anticancer Drug Screen by Susan L. Holbeck and Julian Simon Chapter 13: Yeas as a Model to Study the Immunosuppressive and Chemotherapeutic Drug Rapamycin by John R. Rohde, Sara A. Zurita-Martinez, and Maria E. Cardenas Chapter 14: Use of Yeast as a Model of System for Identifying and Studying Anti-Cancer Drugs by Jun O. Liu and Julian A. Simon Chapter 15: Genetic Analysis of CisplatinResistance in Yeast and Mammals by Seiko Ishida and Ira Herskowitz Chapter 16: Using Yeast Tools to Dissect the Action of Anti-Cancer Drugs: Mechanisms of Enzyme Inhibition and Cell Killing by Agents Targeting DNA Topoisomerases by Anna Rogojina, David Li, Karin C. Nitiss, and John L. Nitiss
