Beschreibung
In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.
Autorenporträt
InhaltsangabePart I: Introduction & Historical Background 1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease Ana Olivera and Juan Rivera Part II: Obtaining the Cells: Purification, Culture, Counting, and Cell Lines 2. Purification of Basophils from Peripheral Human Blood Franco H. Falcone and Bernhard F. Gibbs 3. Mast Cell Purification Protocols Jasmine Farrington, Elizabeth P. Seward, and Peter T. Peachell 4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells Hans Jürgen Hoffmann 5. Generation of Mast Cells from Murine Stem Cell Progenitors Emily J. Swindle 6. Integration of the Human Dermal Mast Cell into the Organotypic Coculture Skin Model Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc 7. The Absolute Basophil Count Elena Borzova and Clemens A. Dahinden 8. Detection of Mast Cells and Basophils by Immunohistochemistry Andrew F. Walls and Cornelia Amalinei 9. Mast Cell and Basophil Cell Lines: A Compendium Egle Passante Part III: Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters 10. Measuring Histamine and Cytokine Release from Basophils and Mast Cells Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov 11. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo 12. Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis Monika Bambousková, Zuzana Hájková, Pavel Dráber, and Petr Dráber 13. Use of Humanized Rat Basophil Leukaemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone 14. Gene Silencing Approaches in Mast Cells and Primary Human Basophils Vadim V. Sumbayev and Bernhard F. Gibbs 15. Basophil Stimulation and Signaling Pathways Edward F. Knol and Bernhard F. Gibbs 16. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, and Luís Escribano Part IV: Whole Organism: Disease and Knockout Models 17. Identification of Murine Basophils by Flow Cytometry and Histology Christian Schwartz and David Voehringer 18. Mast Cell Mediated Reactions In Vivo Vladimir Andrey Giménez-Rivera, Martin Metz, and Frank Siebenhaar
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE
E-Mail: juergen.hartmann@springer.com
