Beschreibung
InhaltsangabePart I. Characterizing the Genome 1. Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei Emily E. Hare and J. Spencer Johnston 2. Chromosome Analysis in Invertebrates and Vertebrates David M. Rowell, Shu Ly Lim and Frank Grutzner 3. Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter 4. Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter Part II. Targeting Regions of the Genome 5. Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing Jeffrey M. Good 6. Accessing the Transcriptome: How to Normalize mRNA Pools Heiko Vogel and Christopher W. Wheat 7. Transcriptome Sequencing Goals, Assembly, and Assessment Christopher W. Wheat and Heiko Vogel 8. Rapid Retrieval of DNA Target Sequences by Primer Extension Capture Adrian W. Briggs Part III. Measuring Genetic Diversity 9. SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric Johnson, and William A. Cresko 10. DNA Microarray-based Mutation Discovery and Genotyping David Gresham 11. Genotyping with Sequenom Martina Bradic, João Costa, and Ivo M. Chelo 12. Isolating Microsatellite Loci: Looking Back, Looking Ahead José A. Andrés and Steven M. Bogdanowicz 13. Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector Amy A. Caudy Part IV. Obtaining Candidate Gene Sequences14. Identification of Homologous Gene Sequences by PCR with Degenerate Primers Michael Lang and Virginie Orgogozo 15. Characterizing cDNA Ends by Circular RACE Patrick T. McGrath 16. Identification of DNA Sequences that Flank a Known Region by Inverse PCR Anastasios Pavlopoulos Part V. Analyzing Candidate Gene Transcripts17. Quantification of Transcript Levels with Quantitative RT-PCR Karen L. Carleton 18. Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences Patricia J. Wittkopp 19. Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern Ryo Futahashi 20. Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning Scott Rifkin Part VI. Testing Candidate Genes and Candidate Mutations 21. Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution Mark Rebeiz and Thomas M. Williams 22. Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution Jay F. Storz and Anthony J. Zera 23. Making Reporter Gene Constructs to Analyze Cis-regulatory Elements José Bessa and José Luis Gómez-Skarmeta 24. PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker's Yeast Erik C. Andersen 25. Production
Inhaltsverzeichnis
Part I. Characterizing the Genome 1. Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei Emily E. Hare and J. Spencer Johnston 2. Chromosome Analysis in Invertebrates and Vertebrates David M. Rowell, Shu Ly Lim and Frank Grutzner 3. Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter 4. Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter Part II. Targeting Regions of the Genome 5. Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing Jeffrey M. Good 6. Accessing the Transcriptome: How to Normalize mRNA Pools Heiko Vogel and Christopher W. Wheat 7. Transcriptome Sequencing Goals, Assembly, and Assessment Christopher W. Wheat and Heiko Vogel 8. Rapid Retrieval of DNA Target Sequences by Primer Extension Capture Adrian W. Briggs Part III. Measuring Genetic Diversity 9. SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric Johnson, and William A. Cresko 10. DNA Microarray-based Mutation Discovery and Genotyping David Gresham 11. Genotyping with Sequenom Martina Bradic, João Costa, and Ivo M. Chelo 12. Isolating Microsatellite Loci: Looking Back, Looking Ahead Jose A. Andres and Steven M. Bogdanowicz 13. Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector Amy A. Caudy Part IV. Obtaining Candidate Gene Sequences 14. Identification of Homologous Gene Sequences by PCR with Degenerate Primers Michael Lang and Virginie Orgogozo 15. Characterizing cDNA Ends by Circular RACE Patrick T. McGrath 16. Identification of DNA Sequences that Flank a Known Region by Inverse PCR Anastasios Pavlopoulos Part V. Analyzing Candidate Gene Transcripts 17. Quantification of Transcript Levels with Quantitative RT-PCR Karen L. Carleton 18. Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences Patricia J. Wittkopp 19. Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern Ryo Futahashi 20. Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning Scott Rifkin Part VI. Testing Candidate Genes and Candidate Mutations 21. Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution Mark Rebeiz and Thomas M. Williams 22. Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution Jay F. Storz and Anthony J. Zera 23. Making Reporter Gene Constructs to Analyze Cis-regulatory Elements Jose Bessa and Jose Luis Gomez-Skarmeta 24. PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker¿s Yeast Erik C. Andersen 25. Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgensis Radoslaw Kamil Ejsmont, Maria Bogdanzaliewa, Kamil Andrzej Lipinski, and Pavel Tomancak 26. Recombination-mediated Genetic Engineering of Lar ...
