Genetically Incorporated Non-Canonical Amino Acids

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Methods and Protocols, Methods in Molecular Biology 2676

ISBN: 1071632507
ISBN 13: 9781071632505
Herausgeber: Yu-Hsuan Tsai/Simon J Elsässer
Verlag: Springer Verlag GmbH
Umfang: xi, 286 S., 1 s/w Illustr., 286 p. 1 illus.
Erscheinungsdatum: 06.06.2023
Auflage: 1/2023
Produktform: Gebunden/Hardback
Einband: GEB

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Artikelnummer: 8788183 Kategorie:

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