Beschreibung
In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.
Autorenporträt
InhaltsangabePart I: BASIC PSEUDOMONAS FEATURES1. Gene Transfer: TransductionEmanuela Frangipani2. Gene Transfer: Transformation/ElectroporationFrédéric Cadoret, Chantal Soscia and Romé Voulhoux3. Gene transfer: ConjugationThibault G. Sana, Aurélie Laubier and Sophie Bleves4. Pseudomonas Bacteriophage Isolation and ProductionJoana Azeredo, Sanna Sillankorva, and Diana Priscilla Pires5. Genotyping MethodsBurkhard Tuemmler6. Drug Susceptibility Testing by Dilution MethodsKaty Jeannot & Patrick Plesiat7. Plate-based Assay for Swimming Motility in Pseudomonas aeruginosaDae-Gon Ha, Sherry L. Kuchma & George A. O'Toole8. Plate-based Assay for Swarming Motility in Pseudomonas aeruginosaDae-Gon Ha, Sherry L. Kuchma and George A. O'Toole9. Motility Assay: Twitching MotilityLynne Turnbull and Cynthia B. Whitchurch10. Qualitative and Quantitative Assays for Flagellum-mediated ChemotaxisJosé-Antonio Reyes-Darias, Cristina García-Fontana, Andrés Corral Lugo, Miriam Rico-Jiménez & Tino Krell11. Microscopic Analysis: Morphotypes and Cellular AppendagesOlga Zaborina, John Alverdy, Megha Shah, and Yimei Chen Part II: PROTEINS AND PROTEIN TRANSPORT12. Determination of Lipolytic ActivitiesKarl-Erich Jaeger and Filip Kovacic13. Elastinolytic and Proteolytic EnzymesContributors: Efrat Kessler and Mary Safrin 14. In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted ProteinsStéphanie L. Rolsma & Dara W. Frank15. Cell FractionationBérengère Ize, Véronique Viarr, and Romé Voulhoux16. Characterization of Molecular Interactions using Isothermal Titration Calorimetry Tino Krell, Jesús Lacal, Cristina Garcia-Fontana, Hortencia Silva-Jimenez, Miriam Rico-Jimenez, Andrés Corral Lugo, Jose A. Reyes Darias, and Juan Luis Ramos 17. Proteomic AnalysisPascal Cosette and Thierry Jouenne18. Membrane Proteomics of Pseudomonas aeruginosaZofia Magnowska, Isabel Hartmann, Lothar Jänsch and Dieter Jahn 19. Construction of Pseudomonas Two-hybrid Libraries for High-throughput AssaysSophie de Bentzmann and Christophe BordiPart III: SMALL MOLECULES AND POLYSACCHARIDES20. Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal MoleculesMatthew Fletcher, Miguel Camara, David A. Barrett, and Paul Williams21. LC-MS/MS Quantitative Analysis of Quorum Sensing Signal MoleculesCatherine A. Ortori, Nigel Halliday, Miguel Camara, Paul Williams, and David A. Barrett22. LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMPYasuhiko Irie and Matthew R. Parsek23. Metabolic Footprinting: Extracellular Metabolomic AnalysisVolker Behrends, Huw D. Williams, and Jaco G. Bundy24. Pyoverdine and Pyochelin MeasurementsFrançoise Hoegy, Gaetan L. A. Mislin & Isabelle J. Schalk25. Measurement of Phenazines in Bacterial CulturesSuzanne E. Kern, and Dianne K. Newman26. Extraction and Measurement of NAD(P)+ and NAD(P)HSuzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman27. Cyanide Measurements in Bacterial Culture and Sputum Chandrika Goh Nair, Ben Ryall, and Huw D. Williams28. Monitoring Iron Uptake by SiderophoresFrançoise Hoegy, and Isabelle J. Schalk29. Exopolysaccharide QuantificationIrina Sadovskaya30. Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of RhamnolipidsAhmad Mohammad Abdel-Mawgoud, François Lépine & Eric Déziel31. LPS quantitation ProceduresJoseph S. Lam, Erin M. Anderson, and Youai Hao 32. Monitoring Lectin Interaction with CarbohydratesSophie de Bentzmann, Annabelle Varrot, and Anne ImbertyPart IV: PSEUDOMON
