Detection of Cryptosporidium species from stool specimens

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39,90 

ISBN: 3659640395
ISBN 13: 9783659640391
Autor: Jad, Beena/Pottathil, Shinu/Grover, PremSingh
Verlag: LAP LAMBERT Academic Publishing
Umfang: 76 S.
Erscheinungsdatum: 23.12.2014
Auflage: 1/2014
Format: 0.5 x 22 x 15
Gewicht: 131 g
Produktform: Kartoniert
Einband: Kartoniert
Artikelnummer: 7690317 Kategorie:

Beschreibung

A total of 500 stool samples were subjected to examination with the help of direct microscopy and concentration methods. Of these, fifty two (10.4%) specimens were positive for Cryptosporidium parvum. Among direct microscopy methods - direct wet mount, negative staining and modified Ziehl - Neelsen staining showed a positivity of 22 (4.4%), 14 (2.8%) and 39 (7.8%) respectively. After the Formol - ether Concentration (FEC) technique, wet mount, negative staining and modified Ziehl - Neelsen stain were positive in 32 (6.4%), 37 ( 7.4%) and 52 (10.4%) cases respectively. Wet mount after Saturated Sodium Chloride Salt Flotation (SSSF) technique was positive in only 6 (1.2%) cases. Modified Ziehl - Neelsen stain after SSSF technique was positive in 19 (3.8%) cases while negative staining after SSSF technique was positive in only 1 (0.2%) case. Cryptosporidium parvum positivity was 1.18 times higher in males as compared to females. The results indicate that the modified Ziehl - Neelsen staining after FEC technique to detect Cryptosporidium parvum in the stool specimens has significantly higher accuracy (p<0.001) and reliability as compared to all the other techniques

Autorenporträt

Dr. Beena Jad is currently working as senior resident in the Department of Microbiology, Govt. Medical College, Jammu.This book shares her experiences in the detection techniques of Cryptosporidium parvum from stool specimens

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