Beschreibung
InhaltsangabeTable of Contents Chapter 1 Introduction [REVIEW CHAPTER] Chapter 2 Vectors and strains for expression [REVIEW CHAPTER] Chapter 3 DNAMediated Transformation Chapter 4 Rational design and optimization of fedbatch and continuous fermentations Chapter 5 Overexpression of a hookworm secretory protein saturates secretory capacity Chapter 6 Initial processing and purification strategies Chapter 7 Rapid screening of chromatography resins for the purification of proteins Chapter 8 Characterization of Olinked saccharides on glycoproteins Chapter 9 Modification of the Nglycosylation pathway to produce homogeneous humanlike glycans using GlycoSwitch plasmids Chapter 10 Nlinked glycan characterization of heterologous proteins Chapter 11 Heavy labeling of recombinant proteins Chapter 12 Selenomethionine labeling of recombinant proteins Chapter 13 Deuterium labeling of proteins Chapter 14 Classical genetic analysis Chapter 15 Identification of pexophagy genes by Restriction EnzymeMediated Integration (REMI) Chapter 16 Characterization of proteinprotein interactions Chapter 17 Localization of proteins and organelles using fluorescence microscopy Chapter 18 Fluorescence microscopy and thinsection electron microscopy
Inhaltsverzeichnis
Table of Contents Chapter 1 - Introduction [REVIEW CHAPTER] Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER] Chapter 3 - DNA-Mediated Transformation Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity Chapter 6 - Initial processing and purification strategies Chapter 7 - Rapid screening of chromatography resins for the purification of proteins Chapter 8 - Characterization of O-linked saccharides on glycoproteins Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids Chapter 10 - N-linked glycan characterization of heterologous proteins Chapter 11 - Heavy labeling of recombinant proteins Chapter 12 - Selenomethionine labeling of recombinant proteins Chapter 13 - Deuterium labeling of proteins Chapter 14 - Classical genetic analysis Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI) Chapter 16 - Characterization of protein-protein interactions Chapter 17 - Localization of proteins and organelles using fluorescence microscopy Chapter 18 - Fluorescence microscopy and thin-section electron microscopy