Beschreibung
In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.
Inhaltsverzeichnis
Part I: dna and rna Based Methodology.PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis,Shelley A. Cole and James E. Hixson. Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis,Jose M. Ordovas. Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method,William H. Karge, III, Ernst J. Schaefer, and Jose M. Ordovas. Generation of Transgenic Mice, Marten H. Hofker and Marco Breuer. Preparation of Knockout Mice, Jesús Osada and Nobuyo Maeda. Part II.Protein Based Methodology.Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins,Jose M. Ordovas. Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography,Jose M. Ordovas and Doreen Osgood. Separation of Polipoproteins by Polyacrylamide Gel Electrophoresis,Jose M. Ordovas. Isolation and Purification of Serum Lipoprotrein (a) Using Lectin Affinity,Leo J. Seman, Carl de Luca, and Jennifer L. Jenner. Electrophoretic Separation of LDL and HDL Subclasses David L. Rainwater. Measurement of Lipoprotein Particles,Catherine Fievet and Jean-Charles Fruchart. Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins,Wolfgang Sattler, Ernst Malle, and Gert M. Kostner. Separation and Quantitation of Phospholipid Classes by HPLC,George M. Patton and Sander J. Robins. Assays of Lecithin Cholesterol Acyltransferase (LCAT), Milada Dobiasova and Jiri J. Frohlich. Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP),Laurent Lagrost. In Vitro Measurement of Lipoprotein and Hepatic Lipases,Elisabet Vilella and Jorge Joven. Cellular Assays for Lipoprotein Receptors,Susan Acton and Attilio Rigotti . Measurements of Proteoglycan-Lipoprotein Interaction by Gel Mobility Shift Assay,Eva Hurt-Camejo, German Camejo, and Peter Sartipy.
