Beschreibung
High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 "Advanced micropropagation systems". First-hand practical applications are detailed for crops - including ornamentals and trees - using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant - shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation. This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise.
Inhaltsverzeichnis
Contributing Authors Preface 1. General introduction: a personal reflection on the use of liquid media for in vitro culture; Walter Preil I. Bioreactors 2. Application of bioreactor design principles to plant micropropagation; Wayne R. Curtis 3. Bioreactor design for propagation of somatic embryos; Anne Kathrine Hvoslef-Eide, Odd Arild S. Olsen, Ragnhild Lyngved, Cristel Munster & Petter H. Heyerdahl 4. Practical aspects of bioreactor application in mass propagation of plants; S. Takayama & M. Akita 5. Simple bioreactors for mass propagation of plants; Meira Ziv 6. Application of bioreactor systems for large scale production of horticultural and medicinal plants; K.Y. Paek, Debasis Chakrabarty & E.J. Hahn 7. Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor; Seppo Sorvari, Riitta Mäkeläinen, Katriina Ahanen & Otto Toldi 8. Cost-effective mass cloning of plants in liquid media using a novel growtek bioreactor; Satyahari Dey 9. Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems; Eun-Joo Hahn & Kee-Yoeup Paek 10. Control of growth and differentiation of bioreactor cultures of Physcomitrella by environmental parameters; Annette Hohe & Ralf Reski II. Temporary Immersion Systems 11. Temporary immersion system: a new concept for use liquid medium in mass propagation; M. Berthouly & H. Etienne 12. Mass propagation of tropical crops in temporary immersion systems; Elio Jimenez Gonzalez 13. Use of growth retardants for banana (Musa AAA cv. Grand Naine) shoot multiplication in temporary immersion systems; Nilca Albany, Elio Jimenez, Jorge Vilchez, Leyanis GarcÃa, Manuel De Feria, Naivy Perez, Zoe SarrÃa, Blanca Perez & Justo Clavelo 14. Somatic embryo germination of Psidium guajava L. in the Rita temporary immersion system and on semisolid medium; Rafael Gomez Kosky, J. Vilchez Perozo, N. Albany Valero & D. Agramonte Peñalver 15. Application of a temporary immersion system in mass propagation of Phalaenopsis; Tino Hempfling & Walter Preil 16. Propagation of Prunus and Malus by temporary immersion; C. Damiano, S.R. La Starza, S. Monticelli, A. Gentile, E. Caboni & A. Frattarelli 17. Optimisation of growing conditions for the apple rootstock M26 grown in RITA containers using temporary immersion principle; Li-Hua Zhu, Xue-Yuan Li & Margareta Welander 18. Experimental use of a novel temporary immersion system for liquid culture of olive microshoots; Katerina Grigoriadou, Miltiadis Vasilakakis, Theofilos Tzoulis & Eleftherios P. Eleftheriou 19. Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems; Ch. Wawrosch, A. Kongbangkerd, A. Köpf & B. Kopp III. Somatic Embryogenesis And Shoot Initiation 20. Propagation of Norway spruce via somatic embryogenesis; Sara von Arnold, Peter Bozhkov, David Clapham, Julia Dyachok, Lada Filonova, Karl-Anders Högberg, Mathieu Ingouff & Malgorzata Wiweger 21. Norway spruce somatic embryogenesis: membrane rafts as a compromise between liquid and solidified media; M. Vagner, Z. Vondrakova, L. Fischerova & J. Opatrna 22. Picea abies somatic embryo development from suspension cultures and agar-based cultures: a comparison; Christopher Hunter & Lionel Levy 23. Somatic embryogenesis by liquid culture of epidermal layers in sunflower: from genetic control to cell development; Michel Petitprez, A. Sarrafi, E. Flores-Berrios, X. Xuhan, C. Briere & L. Gentzbittel 24. Development of photoautotrophy in Coffea somatic embryos enables mass production of clonal transplants; F. Afreen, S.M.A. Zobayed & T. Kozai 25. Potential of flow cytometry for monitoring genetic stability of banana embryogenic cell suspension cultures; Nicolas Roux, H ...
