High Throughput Phenotyping in Plants

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106,99 

Methods and Protocols, Methods in Molecular Biology 918

ISBN: 1617799947
ISBN 13: 9781617799945
Herausgeber: Jennifer Normanly
Verlag: Humana Press
Umfang: xi, 365 S., 14 s/w Illustr., 46 farbige Illustr., 365 p. 60 illus., 46 illus. in color. With online files/update.
Erscheinungsdatum: 15.08.2012
Auflage: 1/2012
Format: 2.5 x 26.5 x 18.5
Gewicht: 867 g
Produktform: Gebunden/Hardback
Einband: GEB

Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High-Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High-Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.

Artikelnummer: 3287865 Kategorie:

Beschreibung

Inhaltsangabe1. Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis            Benjamin J. Cole and Joanne Chory 2. High Throughput Phenotyping of Plant Shoots            Bettina Berger, Bas de Regt, and Mark Tester 3. High Throughput Phenotyping of Root Growth Dynamics            Nima Yazdanbakhsh and Joachim Fisahn 4. LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms            Charles A. Price 5. Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach            Roland Pieruschka, Denis Klimov, Joseph A. Berry, C. Barry Osmond, Uwe Rascher, and Zbigniew S. Kolber 6. Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content            László Sass, Petra Majer, and Éva Hideg 7. High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field            Tim Brown, Christopher Zimmermann, Whitney Panneton, Nina Noah, and Justin Borevitz 8. High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant            Raju Naik Vankudavath, Reddaiah Bodanapu, Yellamaraju Sreelakshmi, and Rameshwar Sharma 9. High Throughput Fractionation of Natural Products for Drug Discovery            Ying Tu and Bing Yan 10. Conducting Molecular Biomarker Discovery Studies in Plants            Christian Schudoma, Matthias Steinfath, Heike Sprenger, Joost T. van Dongen, Dirk Hincha, Ellen Zuther, Peter Geigenberger, Joachim Kopka, Karin Köhl, and Dirk Walther 11. Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry            Mikiko Kojima and Hitoshi Sakakibara 12. Qualitative and Quantitative Screening of Amino Acids in Plant Tissues            Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, and Jerry D. Cohen 13. Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis            Thilani Samarakoon, Sunitha Shiva, Kaleb Lowe, Pamela Tamura, Mary R. Roth, and Ruth Welti 14. ICP-MS as a Tool For High Throughput Analysis of Plants            Javier Seravalli 15. The Plant Volatilome: Methods of Analysis            Carlo Bicchi and Massimo Maffei 16. High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry            David W. Galbraith and Georgina M. Lambert 17. Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta            Shu-Zon Wu and Magdalena Bezanilla 18. A High Throughput Biological Conversion Assay for Determining Lignocellulosic QualityScott J. Lee, Thomas A. Warnick, Susan B. Leschine, and Samuel P. Hazen 19. Carbohydrate Microarrays in Plant Science            Jonatan U. Fangel, Henriette L. Pedersen, Silvia Vidal Melgosa, Louise I. Ahl, Armando Asuncion Salmean, Jack Egelund, Maja Gro Rydahl, Mads H. Clausen, and William G.T. Willats          

Inhaltsangabe1. Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis            Benjamin J. Cole and Joanne Chory 2. High Throughput Phenotyping of Plant Shoots            Bettina Berger, Bas de Regt, and Mark Tester 3. High Throughput Phenotyping of Root Growth Dynamics            Nima Yazdanbakhsh and Joachim Fisahn 4. LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms            Charles A. Price 5. Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach            Roland Pieruschka, Denis Klimov, Joseph A. Berry, C. Barry Osmond, Uwe Rascher, and Zbigniew S. Kolber 6. Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content            László Sass, Petra Majer, and Éva Hideg 7. High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field            Tim Brown, Christopher Zimmermann, Whitney Panneton, Nina Noah, and Justin Borevitz 8. High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant            Raju Naik Vankudavath, Reddaiah Bodanapu, Yellamaraju Sreelakshmi, and Rameshwar Sharma 9. High Throughput Fractionation of Natural Products for Drug Discovery            Ying Tu and Bing Yan 10. Conducting Molecular Biomarker Discovery Studies in Plants            Christian Schudoma, Matthias Steinfath, Heike Sprenger, Joost T. van Dongen, Dirk Hincha, Ellen Zuther, Peter Geigenberger, Joachim Kopka, Karin Köhl, and Dirk Walther 11. Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry            Mikiko Kojima and Hitoshi Sakakibara 12. Qualitative and Quantitative Screening of Amino Acids in Plant Tissues            Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, and Jerry D. Cohen 13. Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis            Thilani Samarakoon, Sunitha Shiva, Kaleb Lowe, Pamela Tamura, Mary R. Roth, and Ruth Welti 14. ICP-MS as a Tool For High Throughput Analysis of Plants            Javier Seravalli 15. The Plant Volatilome: Methods of Analysis            Carlo Bicchi and Massimo Maffei 16. High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry            David W. Galbraith and Georgina M. Lambert 17. Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta            Shu-Zon Wu and Magdalena Bezanilla 18. A High Throughput Biological Conversion Assay for Determining Lignocellulosic QualityScott J. Lee, Thomas A. Warnick, Susan B. Leschine, and Samuel P. Hazen 19. Carbohydrate Microarrays in Plant Science            Jonatan U. Fangel, Henriette L. Pedersen, Silvia Vidal Melgosa, Louise I. Ahl, Armando Asuncion Salmean, Jack Egelund, Maja Gro Rydahl, Mads H. Clausen, and William G.T. Willats          

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