Rapid Cycle Real-Time PCR Methods and Applications

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ISBN: 3642623174
ISBN 13: 9783642623172
Herausgeber: Carl Wittwer/Meinhard Hahn/Karen Kaul
Verlag: Springer Verlag GmbH
Umfang: vii, 223 S., 15 s/w Illustr., 511 farbige Illustr., 223 p. 526 illus., 511 illus. in color.
Erscheinungsdatum: 23.10.2012
Auflage: 1/2012
Produktform: Kartoniert
Einband: Kartoniert

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybrdidization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Artikelnummer: 7048024 Kategorie:

Beschreibung

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

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Springer Verlag GmbH
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69121 Heidelberg
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E-Mail: juergen.hartmann@springer.com

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