Beschreibung
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Inhaltsverzeichnis
InhaltsangabePart I. Basic Methodology New Rapid Multicolor PRINS Protocol Ju Yan, Macoura Gadji, Kada Krabchi, and Régen Drouin PRINS Combined With Peptide Nucleic Acid Labeling Franck Pellestor and Petra Paulasova PRINS Combined With Indirect Immunofluorescence Philippe Coullin PRINS for the Detection of Unique Sequences Stephen S. Wachtel and Avirachan T. Tharapel SingleCopy and Point Mutation Analysis (RollingCircle PRINS) Jørn Koch Analysis of Sperm Aneuploidy by PRINS Franck Pellestor, Brigitte Andréo, Jacques Puechberty, Geneviève Lefort, and Pierre Sarda In Situ PCR Protocols Omar Bagasra and Twaina Harris Part II. Research and Clinical Applications Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS Jørn Koch PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei Antonella Russo PRINS for the Detection of Gene Deletions in Cancer Avirachan T. Tharapel and Stephen S. Wachtel Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene Liborio Stuppia, Dario La Sala, and Caterina Cinti Brain Tissue Preparations for Chromosomal PRINS Labeling Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, and Yuri B. Yurov PRINS on Plant Chromosomes Jirí Macas, Alice Navrátilová, Marie Kubaláková, and Jaroslav Dolezel DualColor PRINS for In Situ Detection of Fetal Cells in Maternal Blood Kada Krabchi, Macoura Gadji, Ju Yan, and Régen Drouin PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos Franck Pellestor, Brigitte Andréo, Tal Anahory, and Samir Hamamah Microdissection With PCR In Situ Allen T. Christian and ChristineHara Application of Reverse Transcription In Situ PCR in Cancer Analysis Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, and Yuji Ohtsuki In Situ Reverse Transcription PCR on Plant Tissues Zbigniew Przybecki, Ewa Siedlecka, Marcin Filipecki, and Ewa Urbanczyk-Wochniak In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection Barry Slobedman Development and Adaptation of the PRINS Technology: An Overview Franck Pellestor Latest Developments in In Situ PCR Omar Bagasra and Twaina Harris Index
