Peptide Synthesis and Applications

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106,99 

Methods in Molecular Biology 298

ISBN: 1588293173
ISBN 13: 9781588293176
Herausgeber: John Howl
Verlag: Humana Press
Umfang: x, 262 S., 69 s/w Illustr., 262 p. 69 illus.
Erscheinungsdatum: 28.02.2005
Auflage: 1/2005
Produktform: Gebunden/Hardback
Einband: GEB

Peptide synthesis provides novel materials that can serve as both valuable research tools and candidate therapeutics. In Peptide Synthesis and Applications, a panel of expert researchers and established group leaders describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate numerous applications that employ peptides as unique and essential materials. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Stateoftheart and highly practical, Peptide Synthesis and Applications offers novice and experienced investigators alike powerful techniques for the design and manufacture of their own peptidebased materials that can be used as productive research tools or developed as promising new therapeutics.

Artikelnummer: 5791410 Kategorie:

Beschreibung

InhaltsangabePart I: Common Strategies Fundamentals of Modern Peptide Synthesis Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, and Gilles Subra Chimerism: A Strategy to Expand the Utility and Applications of Peptides John Howl Part II: Synthetic Methodologies and Applications Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars Joanne T. Blanchfield and Istvan Toth Synthesis of Linear, Branched, and Cyclic Peptide Chimera Gábor Mezö and Ferenc Hudecz Synthesis of Cell-Penetrating Peptides for Cargo Delivery Margus Pooga and Ülo Langel Incorporation of the Phosphotyrosyl Mimetic 4(Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides ZhuJun Yao, Kyeong Lee, and Terrence R. Burke, Jr. Expressed Protein Ligation for Protein Semisynthesis and Engineering Zuzana Machova and Annette G. Beck-Sickinger Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides Kalle Kilk and Ülo Langel Quenched Fluorescent Substrate-Based Peptidase Assays Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, and A. Ian Smith A Convenient Method for the Synthesis of Cyclic Peptide Libraries Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton, Andreas Rhümann, Stephen G. Love, Tran T. Tran, and Mark L. Smythe HighThroughput Peptide Synthesis Michal Lebl and John Hachmann Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C-Terminal Modified Peptides Jordi Alsina, Steven A. Kates, George Barany, and Fernando Albericio Synthesis of Peptide Bioconjugates Ferenc Hudecz Part III: Practical Guides Protein Identification by Mass Spectrometric Analyses of Peptides AshleyMartin Manual SolidPhase Synthesis of Glutathione Analogs: A LaboratoryBased Short Course Ursel Soomets, Mihkel Zilmer, and Ülo Langel Index

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