Beschreibung
Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.
Autorenporträt
InhaltsangabePart I: Reactive Oxygen and Nitrogen Techniques 1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS Ivana Milic and Maria Fedorova 2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang 3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors Detao Gao, Larry W. Sayre, and Eugene A. Podrez 4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA Takayuki Shibamoto 5. Nitric Oxide Availability as a Marker of Oxidative Stress Dan Pierini and Nathan S. Bryan 6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay Ingrit Hamann and Andrea Hartwig 7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases Ernst H. Oliw and Anneli Wennman 8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H2S Repair Zhangrong Lou, Peng Li, and Keli Han 9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted 10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis García-Fuentes 11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell Tadeusz Malinski Part II: Antioxidant Technology and Application 12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal 13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity Ilya Pinchuk and Dov Lichtenberg 14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements Eric Deconinck, Deborah Custers, and Jacques Omer De Beer 15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector Muhammad Imran Khan, Zafar Iqbal, and Abad Khan 16. Portable Nanoparticle Based Sensors for Antioxidant Analysis Erica Sharpe and Silvana Andreescu 17. Fe3+-Fe2+ Transformation Method: An Important Antioxidant Assay Ilhami Gülçin 18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu 19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins Ricardo A. Mosquera, Laura Estévez, and Mercedes García-Bugarín 20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples Marcela A. Segundo, Ildikó V. Tóth, Luís M. Magalhães, and Salette Reis 21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific Protein Resi
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE
E-Mail: juergen.hartmann@springer.com
