Towards Clearer Imaging of Luminescent and Bioluminescent Samples

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Increasing Background Rejection in Luminescent and Bioluminescent Imaging Using Optical and Computational Techniques

ISBN: 384438796X
ISBN 13: 9783844387964
Autor: Mader, Kevin
Verlag: LAP LAMBERT Academic Publishing
Umfang: 68 S.
Erscheinungsdatum: 22.06.2011
Auflage: 1/2011
Format: 0.5 x 22 x 15
Gewicht: 119 g
Produktform: Kartoniert
Einband: KT
Artikelnummer: 1436802 Kategorie:

Beschreibung

Bioluminescence has become an excellent method for tagging and easily identifying biochemical processes. The process produces light from a set of biochemical reactions such that a light source is not needed and imaging can be done in regions a light source could not easily be placed. Also larger samples do not require any scanning, as other techniques like both confocal and two-photon. The major issue with the technique is that because it does not require illumination, the resulting images are usually blurry and poorly sectioned. In this thesis a series of optical and computational techniques are used to take advantage of the propagation of phase rather than intensity to reject significantly more light from out-side the focal region.

Autorenporträt

Kevin Mader studied Electrical Engineering and Photonics at Boston University specializing in biomedical applications. During his studies, he did a summer fellowship at the Center for Biophotonics at the University of California Davis, and an international research exchange at the Department of Physics at Universität Bielefeld in Germany.

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