Beschreibung
This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.
Autorenporträt
InhaltsangabePart I: In Vitro Models of the Hepatocyte Life Cycle1. Isolation And Culture Of Mouse Hepatocytes: Gender-Specific Gene Expression Responses To Chemical TreatmentsLyndsey Houseman, Mina Edwards, Ian R. Phillips, and Elizabeth A. Shephard 2. Cryopreservation of HepatocytesNicola J. Hewitt and Albert P. Li 3. Culture Conditions Promoting Hepatocyte Proliferation And Cell Cycle SynchronizationAnne Corlu and Pascal Loyer 4. Immortalized Human Hepatic Cell Lines For In Vitro Testing And Research PurposesEva Ramboer, Tamara Vanhaecke, Vera Rogiers, and Mathieu Vinken 5. Culture and Functional Characterization Of Human Hepatoma Hepg2 CellsMaría Teresa Donato, Laia Tolosa, and María José Gómez-Lechón 6. Establishment And Characterization Of An In Vitro Model Of Fas-Mediated Hepatocyte Cell DeathMathieu Vinken, Michaël Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, and Vera Rogiers 7. Serum-Free Directed Differentiation Of Human Embryonic Stem Cells To HepatocytesKate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, and David C. Hay 8. Human Skin-Derived Precursor Cells: Isolation, Expansion And Hepatic DifferentiationJoery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers 9. Generation Of Hepatocytes From Pluripotent Stem Cells For Drug Screening and Developmental ModelingRichard L. Gieseck III, Ludovic Vallier, and Nicholas R.F. Hannan 10. Differentiation-Promoting Medium Additives For Hepatocyte Cultivation and Cryopreservation Varvara Gouliarmou, Olavi Pelkonen, and Sandra Coecke 11. Co-Culture and Long-Term Maintenance Of HepatocytesMerav Cohen, Gahl Levy, and Yaakov Nahmias 12. Primary Hepatocytes In Sandwich CultureJanneke Keemink, Marlies Oorts, and Pieter Annaert 13. Establishing Liver Bioreactors For In Vitro ResearchSofia P. Rebelo, Rita Costa, Marcos F.Q. Sousa, Catarina Brito, and Paula M. Alves 14. Epigenetic Modifications As Anti-Dedifferentiation Strategy For Primary Hepatocytes In CultureJennifer Bolleyn, Joanna Fraczek, Vera Rogiers, and Tamara Vanhaecke 15. Transfection Of Primary Hepatocytes With Liver-Enriched Transcription Factors Using Adenoviral VectorsMarta Benet, Ramiro Jover, and Roque Bort Part II: In Vitro Methods to Probe Hepatocyte Functionality and Toxicity 16. Transcriptomics Of Hepatocytes Treated With Toxicants For Investigating Molecular Mechanisms Underlying HepatotoxicityVaibhav Shinde, Regina Stöber, Harshal Nemade, Isaia Sotiriadou, Jürgen Hescheler, JanHengstler, and Agapios Sachinidis 17. Global Microrna Analysis In Primary Hepatocyte CulturesJulian Krauskopf, Almudena Espín Pérez, Jos C. Kleinjans, and Theo M. de Kok 18. Mass Spectrometry-Based Proteomics For Relative Protein Quantification And Biomarker Identification In Primary Human HepatocytesLisa Dietz and Albert Sickmann 19. Targeted Metabolomics For Homocysteine-Related Metabolites In Primary HepatocytesIrena Selicharová and Marek Korínek 20. Measurement Of Cytochrome P450 Enzyme Induction And Inhibition In Human Hepatoma CellsRobim M. Rodrigues, Joery De Kock, Tatyana Doktorova, Vera Rogiers, and Tamara Vanhaecke 21. Analysis Of Sinusoidal Drug Uptake Transporter Activities In Primary Human HepatocytesMarc Le Vée, Elodie Jouan, Claire Denizot, Yannick Parmentier, and Olivier Fardel 22. Measurement Of Albumin Secretion As Functionality Test In Primary Hepatocyte CulturesKarolien Buyl, Joery De Kock, Jennifer Bolleyn,
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
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14197 Berlin
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E-Mail: juergen.hartmann@springer.com
