Systemic Lupus Erythematosus

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106,99 

Methods and Protocols, Methods in Molecular Biology 1134

ISBN: 149390325X
ISBN 13: 9781493903252
Herausgeber: Paul Eggleton/Frank J Ward
Verlag: Humana Press
Umfang: xvii, 263 S., 13 s/w Illustr., 22 farbige Illustr., 263 p. 35 illus., 22 illus. in color.
Erscheinungsdatum: 05.02.2014
Auflage: 1/2014
Produktform: Gebunden/Hardback
Einband: GEB

Systemic Lupus Erythematosus: Methods and Protocols describes a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system, and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols  appeals to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.

Artikelnummer: 5986367 Kategorie:

Beschreibung

Inhaltsangabe1. Pathology of Systemic Lupus Erythematosus - The Challenges AheadPedro Correia Azevedo, Grainne Murphy, David Isenberg2. The Lupus Biomarker Odyssey: One ExperienceJoseph M. Ahearn, Susan Manzi, and Chau-Ching Liu 3. Detection of Anti Nuclear Antibodies in SLEYashwant Kumar4. Tests for Circulating Immune ComplexesMark H. Wener5. Methods for Measuring Antibody Dependent Cell-Mediated Cytotoxicity in vitroAaron S. Miller, Max L. Tejada, and Hélène Gazzano-Santoro6. Recombinant Antibody Microarray for Profiling the Serum Proteome of SLECarl A.K. Borrebaeck, Gunnar Sturfelt, and Christer Wingren 7. Bi-functional Antibody Fragment-based Fusion Proteins for the Targeted Elimination of Pathogenic T-cell SubsetsWijnand Helfrich and Edwin Bremer8. TLC Immunostaining for Detection of "Anti-Phospholipid" AntibodiesFabrizio Conti, Cristiano Alessandri, Francesca Romana Spinelli, Antonella Capozzi, Francesco Martinelli, Serena Recalchi, Roberta Misasi, Guido Valesini, Maurizio Sorice9. Induced Murine Models of Systemic Lupus ErythematosusYuan Xu, Leilani Zeumer, Westley H. Reeves, and Laurence Morel10. Measuring Interferon Alpha and other Cytokines in SLEOlferiev, Mikhail; Lliguicota, Mari; Kirou, Kyriakos A; Crow, Mary K.11. Detection of SLE antigens in Neutrophils Extracellular Traps (NETs)Carmelo Carmona-Rivera and Mariana J. Kaplan12. Detection and Characterization of Autoantibodies Against Modified Self-proteins in SLE Sera after Exposure to Reactive Oxygen and Nitrogen Species Brent J. Ryan and Paul Eggleton 13. Generation of Self-peptides to Treat Systemic Lupus Erythematosus Jean-Paul Briand, Nicolas Schall and Sylviane Muller14. Measurement of Malondialdehyde, Glutathione, and Glutathione Peroxidase in SLE PatientsTamer A. Gheita and Sanaa A. Kenawy 15. Evaluating a Particular Circulating MicroRNA Species from an SLE Patient using Stem Loop qRT-PCRWeiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai16. Microarray Technology for Analysis of MicroRNA Expression in Renal Biopsies of Lupus Nephritis PatientsWeiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai17. Laboratory Tests for the Antiphospholipid SyndromeCharis Pericleous, Vera M.Ripoll, Ian Giles, Yiannis Ioannou18. Isolation, Polarisation, and Expansion of CD4+ T Helper Cell Lines and Clones using Magnetic BeadsLekh N. Dahal, Robert N. Barker, and Frank J. Ward19. Meta-analysis as a Diagnostic Tool for Predicting Disease Onset and/or Activity in Systemic Lupus ErythematosusIsabel Cottrell, Asma Khan, Sidra Maqsood, Jemma Thornes and Paul Eggleton. 

Autorenporträt

Inhaltsangabe1. Pathology of Systemic Lupus Erythematosus - The Challenges AheadPedro Correia Azevedo, Grainne Murphy, David Isenberg2. The Lupus Biomarker Odyssey: One ExperienceJoseph M. Ahearn, Susan Manzi, and Chau-Ching Liu 3. Detection of Anti Nuclear Antibodies in SLEYashwant Kumar4. Tests for Circulating Immune ComplexesMark H. Wener5. Methods for Measuring Antibody Dependent Cell-Mediated Cytotoxicity in vitroAaron S. Miller, Max L. Tejada, and Hélène Gazzano-Santoro6. Recombinant Antibody Microarray for Profiling the Serum Proteome of SLECarl A.K. Borrebaeck, Gunnar Sturfelt, and Christer Wingren 7. Bi-functional Antibody Fragment-based Fusion Proteins for the Targeted Elimination of Pathogenic T-cell SubsetsWijnand Helfrich and Edwin Bremer8. TLC Immuonstaining for Detection of "Anti-Phospholipid" AntibodiesFabrizio Conti, Cristiano Alessandri, Francesca Romana Spinelli, Antonella Capozzi, Francesco Martinelli, Serena Recalchi, Roberta Misasi, Guido Valesini, Maurizio Sorice9. Induced Murine Models of Systemic Lupus ErythematosusYuan Xu, Leilani Zeumer, Westley H. Reeves, and Laurence Morel10. Measuring Interferon Alpha and other Cytokines in SLEOlferiev, Mikhail; Lliguicota, Mari; Kirou, Kyriakos A; Crow, Mary K.11. Detection of SLE antigens in Neutrophils Extracellular Traps (NETs)Carmelo Carmona-Rivera and Mariana J. Kaplan12. Detection and Characterization of Autoantibodies Against Modified Self-proteins in SLE Sera after Exposure to Reactive Oxygen and Nitrogen Species Brent J. Ryan and Paul Eggleton 13. Generation of Self-peptides to Treat Systemic Lupus Erythematosus Jean-Paul Briand, Nicolas Schall and Sylviane Muller14. Measurement of Malondialdehyde, Glutathione, and Glutathione Peroxidase in SLE PatientsTamer A. Gheita and Sanaa A. Kenawy 15. Evaluating a Particular Circulating MicroRNA Species from an SLE Patient using Stem Loop qRT-PCRWeiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai16. Microarray Technology for Analysis of MicroRNA Expression in Renal Biopsies of Lupus Nephritis PatientsWeiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai17. Laboratory Tests for the Antiphospholipid SyndromeCharis Pericleous, Vera M.Ripoll, Ian Giles, Yiannis Ioannou18. Isolation, Polarisation, and Expansion of CD4+ T Helper Cell Lines and Clones using Magnetic BeadsLekh N. Dahal, Robert N. Barker, and Frank J. Ward19. Meta-analysis as a Diagnostic Tool for Predicting Disease Onset and/or Activity in Systemic Lupus ErythematosusIsabel Cottrell, Asma Khan, Sidra Maqsood, Jemma Thornes and Paul Eggleton. 

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