Beschreibung
Gaining an understanding of the mechanisms by which cells process and respond to extracellular cues has become a major goal in many areas of bi- ogy and has attracted the attentions of almost every traditional discipline within the biological sciences. At the heart of these divergent endeavors are common methods that can aid biochemists, physiologists, and pharmacologists in ta- ling the specific questions addressed by their research. In Receptor Signal Transduction Protocols, a diverse array of meth- ologies employed to interrogate ligand--receptor and receptor-effector int- actions are described by authors who have devised and successfully applied them. The authors blend excellent descriptions and applications of fairly well established methodologies with new technologies at the cutting-edge of signal transduction research and as such I hope the present volume will complement and extend a previous excellent volume in this series edited by David Kendall and Stephen Hill (Methods Molecular Biology, vol. 41, Signal Transd- tion Protocols).
Autorenporträt
InhaltsangabeMethods for Characterizing Receptors. RadioligandBinding Methods for Membrane Preparations and Intact Cells Mary Keen SiteDirected Mutagenesis and Chimeric Receptors in the Study of ReceptorLigand Binding Mark E. Olah and Gary L. Stiles Approaches to the Stable Transfection of G Protein-Coupled Receptors Andrea Townsend-Nicholson Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels Hartmut Lüddens and Esa R. Korpi The Generation of Receptor-Selective Antibodies John J. Mackrill In Situ Hybridization Judith C.W. Mak and Peter J. Barnes Immunocytochemical Methods for Investigating Receptor Localization Maria I. Fonseca and R. Dale Brown Methods for Studying Receptor Coupling Mechanisms. Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes Sebastian Lazareno Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding Laura J. Sim, Dana E. Selley, and Steven R. Childers AgonistInduced HighAffinity GTP Hydrolysis as an Index of ReceptorMediated G Protein Activation in Mammalian Brain Membranes Yuji Odagaki and Kjell Fuxe Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling Ethan S. Burstein, Tracy A. Spalding, and Mark R. Brann Identification and Quantitation of G Protein a-Subunits Ian Mullaney and Graeme Milligan Covalent Modification of G Proteins by Affinity Labeling Martin Hohenegger, Michael Freissmuth, and Christian Nanoff Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection Stephen R. Ikeda Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating PhospholipaseC Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, and Günter Schultz Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, and Malcolm P. Caulfield Methods for Investigating the Regulation of Receptor Function Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation Andrew B. Tobin Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation Alison W. Gagnon and Eamonn Kelly Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells Diana M. Slowiejko and Stephen K. Fisher Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription Philip A. Iredale, Maura E. Charlton, John D. Alvaro, and Ronald S. Duman Index.
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
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14197 Berlin
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E-Mail: juergen.hartmann@springer.com
