Beschreibung
InhaltsangabePart I: Overview of Microglia Biology1. A Brief Overview of Multi-Talented MicrogliaBertrand Joseph and Jose Luis Venero Part II: Isolation and Culture of Microglia2. Cell Culturing of Human and Murine Microglia Cell LinesJohanna Rodhe 3. Microglia Isolation from Adult Mouse Brain Jae-Kyung Lee, Malú G Tansey 4. Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat BrainsTomas Deierborg 5. Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation TechnologyAshley S. Harms and Malú G. Tansey 6. Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content AnalysisAmy M. Smith, Hannah M. Gibbons, Claire Lill, Richard L. M. Faull and Mike Dragunow Part III: Depletion and Transduction of Microglia7. Depletion of Microglia from Primary Cellular CulturesLorena Pont-Lezica, Sabrina Colasse and Alain Bessis 8. Lentiviral Transduction of Cultured MicrogliaTakahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh and Kazuhide Inoue Part IV: Analysis of Microglial Cytokine Production9. Microglial Activation: Measurement of Cytokines by Flow CytometryDeepak Kumar Kaushik and AnirbanBasu 10. In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide ProbesBettina Clausen, Christina Fenger and Bente Finsen 11. Use of Meso-scale DiscoveryTM to Examine Cytokinecontentin Microglia Cell SupernatantMiguel A. Burguillos Part V: Analysis of Microglia Activation12. Analysis of Microglial Production of Reactive Oxygen and Nitrogen SpeciesUrteNeniskyte and Guy C. Brown 13. Quantification of Active Caspase-3 and Active Caspase-8 in Microglia CellsEdel Kavanagh 14. Quantification of Microglial Phagocytosis by a Flow Cytometer-based AssayRefik Pul, Chittappen Kandiyil Prajeeth, Martin Stangel 15. Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen 16. Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia Tom Schilling, and Claudia Eder 17. Patch Clamp Protocols to Study Ion Channel Activity in Microglia Tom Schilling, and Claudia Eder Part VI: Analysis of Microglia Polarization18. Studying M1 and M2 States in Adult MicrogliaSadanand M. Gaikwad and Michael T. Heneka 19. Isolating, Culturing, and Polarizing Primary Human Adult and Fetal MicrogliaBryce A. Durafourt, Craig S. Moore, Manon Blain, and Jack P. Antel Part VII: Co-culture Systems to Analysis Microglia Interactions with Other Cell Types20. Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-culturesFernando G Correa, Miriam Hernangómez, and Carmen Guaza 21. Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with MicrogliaShih-Heng Chen, Esteban A. Oyarzabal and Jau-Shyong Hong Part VIII: Analysis of Microglia Functions in vivo22. Microglia Detection by Enzymatic HistochemistryBeatriz Almolda, Berta González and Bernardo Castellano 23. Tomato Lectin Histochemistry for Microglial VisulizationNàdia Villacampa, Beatriz Almolda, Berta González and Bernardo Castellano 24. Immunohistochemical Detection of MicrogliaRocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera 25. Intrathecal Infusion of Microglia CellsTakahiro Masuda, Makoto Tsuda,
