Beschreibung
This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish. Recent advances of experiments using the tetracycline-regulated gene expression and Tol2 transposon systems are also included. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Practical and authoritative, Electroporation Methods in Neuroscience serves to aid scientists in the further study into this crucially important way to study cells.
Autorenporträt
Inhaltsangabe1. In utero Electroporation of the Mouse EmbryoTetsuichiro Saito 2. Exo utero Electroporation of the Mouse EmbryoTetsuichiro Saito 3. In Vivo Electroporation of the Postnatal Rodent ForebrainCamille Boutin and Harold Cremer 4. Electroporation in the Rodent Retina in vivo and in vitroTakahiko Matsuda 5. Electroporation in the Developing Mouse Olfactory BulbFumiaki Imamura and Charles A. Greer 6. Cell Migration Analysis of Cortical Interneurons after ElectroporationFujio Murakami 7. Characterization of Newborn Interneurons In The Mouse Olfactory Bulb Using Postnatal ElectroporationHiroo Takahashi, Sei-ichi Yoshihara, Ryo Asahina, Yoshinori Tamada, and Akio Tsuboi 8. Genetic Manipulation Of Gyrencephalic Carnivoresusing in utero electroporationHiroshi Kawasaki 9. Electroporation for the Chick Embryonic CNSJun Hatakeyama and Kenji Shimamura 10. Targeting the Zebrafish Nervous System using In Vivo ElectroporationJohn H. Horne 11. Gene Transfer into Cultured Mammalian Embryos by ElectroporationMasanori Takahashi,Takako Kikkawa, and Noriko Osumi 12. Electroporation Method for Mammalian CNS Neurons in Organotypic Slice CulturesNaoyuki Matsumoto, Kensuke Sasaki, and Nobuhiko Yamamoto 13. Electroporation of Dissociated Hippocampal NeuronsAtsushi Baba and Tetsuichiro Saito 14. Tol2-Mediated Transgenesis And Its Application To ElectroporationKoichi Kawakami 15. Control of Gene Expression In Neurons by the use of in vivo Electroporation and the Tetracycline System Tatsuya Sato, Yuko Muroyama, and Tetsuichiro Saito
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE
E-Mail: juergen.hartmann@springer.com
