Beschreibung
InhaltsangabePart I. Chemical Probes of Membrane Protein Structure and Function 1. Engineering K+ Channels using Semisynthesis Alexander G. Komarov, Corey A. Costantino, and Francis I. Valiyaveetil 2. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes Zhengmao Hua and William R. Kobertz 3. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules Sviatoslav N. Bagriantsev and Daniel L. Minor Jr. 4. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR HeterodimersJessica H. Harvey, Richard M. van Rijn, and Jennifer L. Whistler Part II. Photochemical Control of Protein and Cellular Function 5. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy Joseph P. Y. Kao and Sukumaran Muralidharan 6. Characterization of One- and Two- Photon Photochemical Uncaging Efficiency Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, and Maurice Goeldner 7. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization Alexandre Mourot, Timm Fehrentz, and Richard H. Kramer 8. A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable LigandsMatthew R. Banghart and Dirk Trauner 9. Developing a Photoreactive Antagonist Pamela M. England Part III. Chemical Probes for Imaging in the Nervous System 10. Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors Laurel A. Cooley, Vladimir V. Martin, and Kyle R. Gee 11. Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation Corey D. Acker and Leslie M. Loew 12. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin ReceptorRuud Hovius 13. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots Qi Zhang 14. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, and Alan Jasanoff
Autorenporträt
InhaltsangabePart I. Chemical Probes of Membrane Protein Structure and Function 1. Engineering K+ Channels using Semisynthesis Alexander G. Komarov, Corey A. Costantino, and Francis I. Valiyaveetil 2. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes Zhengmao Hua and William R. Kobertz 3. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules Sviatoslav N. Bagriantsev and Daniel L. Minor Jr. 4. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers Jessica H. Harvey, Richard M. van Rijn, and Jennifer L. Whistler Part II. Photochemical Control of Protein and Cellular Function 5. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy Joseph P. Y. Kao and Sukumaran Muralidharan 6. Characterization of One- and Two- Photon Photochemical Uncaging Efficiency Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, and Maurice Goeldner 7. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization Alexandre Mourot, Timm Fehrentz, and Richard H. Kramer 8. A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable Ligands Matthew R. Banghart and Dirk Trauner 9. Developing a Photoreactive Antagonist Pamela M. England Part III. Chemical Probes for Imaging in the Nervous System 10. Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors Laurel A. Cooley, Vladimir V. Martin, and Kyle R. Gee 11. Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation Corey D. Acker and Leslie M. Loew 12. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor Ruud Hovius 13. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots Qi Zhang 14. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, and Alan Jasanoff
