Produktbild: Basic Exercises in Immunochemistry

Basic Exercises in Immunochemistry

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A Laboratory Manual

ISBN: 3540094539
ISBN 13: 9783540094531
Autor: Nowotny, A
Verlag: Springer Verlag GmbH
Umfang: xiv, 314 S., 28 s/w Illustr., 314 p. 28 illus.
Erscheinungsdatum: 01.09.1979
Auflage: 2/1979
Produktform: Kartoniert
Einband: Kartoniert

Inhaltsangabe1 Isolations and Preparations.- Exercise No. 1 Fractionation of Serum Proteins by Ammonium Sulfate.- Exercise No. 2 Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Column.- Exercise No. 3 Isolation of IgG by DEAE Cellulose.- A Column Chromatography.- B Batch-Type Operation.- Exercise No. 4 Dissociation of Macroglobulins With 2-Mercaptoethanol.- A.- B.- Exercise No. 5 Enzymatic Cleavage of IgG.- Exercise No. 6 Chromatographic Separation of the IgG Fragments.- Exercise No. 7 Separation and Isolation of H and L Chains of IgG.- Exercise No. 8 Affinity Chromatography and Its Application to Antibody Isolation.- Exercise No. 9 Cellulose Acetate Paper Electrophoresis of Human Serum.- Exercise No. 10 Disc Electrophoresis in Polyacrylamide Gel.- A Separation of Serum Proteins.- B SDS Gel Electrophoresis of Erythrocyte Membrane Proteins.- c Molecular Weight Determination by SDS Gel Electrophoresis.- Exercise No. 11 Separation of Human Hemoglobins by Electro-focusing.- Exercise No. 12 Conjugation of Antibodies With Fluorescein and Rhodamine.- A Fluorescein.- B Rodamine.- Exercise No. 13 Enzymatic Labeling of Proteins With 125I.- A.- B.- Exercise No. 14 Preparation of Peroxidase-Labeled Antibody.- Exercise No. 15 Preparation of Soluble Dinitrophenyl Proteins.- Exercise No. 16 Preparation of Dinitrophenyl- and Trinitrophenyl-Labeled Erythrocytes.- A.- B.- Exercise No. 17 Isolation of Cell Membranes From Ascites Tumors.- Exercise No. 18 Preparation and Use of Synthetic Liposomes.- Exercise No. 19 Isolation of Murine Transplantation Antigen Preparation.- Exercise No. 20 Isolation of Bacterial H-Antigens.- Exercise No. 21 Extraction of Bacterial 0 Antigens (Endotoxins or Lipopolysaccharides).- A Trichloroacetic Acid Method.- B Phenol-Water Procedure.- c Chloroform-Methanol Extraction of Endotoxic Glycolipid (EGL) From Rough Mutants.- Exercise No. 22 Preparation of 14C-Labeled Endotoxin and Endotoxic Glycolipid (EGL).- Exercise No. 23 Isolation of Bacterial Capsular Polysaccharides, K Antigens.- Exercise No. 24 Extraction of Teichoic Acid.- Exercise No. 25 Extraction of Pneumococcus Type XIV Polysaccharide.- Exercise No. 26 Isolation of Phytohemagglutinin.- Exercise No. 27 A and B Blood Group Antigen Preparations From Erythrocytes.- A Solubilization With Dilute NaOH.- B The Use of Detergent.- Exercise No. 28 Isolation of NN Blood Group Antigen From Human Erythrocytes.- Exercise No. 29 Isolation of Forssman Hapten From Erythrocytes.- Exercise No. 30 Isolation of Ragweed Pollen Allergens.- Exercise No. 31 Ultrasonic Disruption of Bacteria and Density-Gradient Centrifugation of the Products.- Exercise No. 32 Vacuum Distillation and Freeze Drying.- 2 Structural Studies.- 2.1 2.1.1 Qualitative Methods, Hydrolytic Procedures.- Exercise No. 33 Determination of Optimal Hydrolytic Conditions.- A Carbohydrate Liberation.- B Liberation of Amino Acids and Amino Sugars.- Exercise No. 34 Hydrolysis of Lipopolysaccharide With Acid.- Exercise No. 35 Partial Hydrolysis of Lipopolysaccharide With Ion Exchanger.- A Discontinuous Method.- B Continuous System.- 2.1.2 Separation and Isolation of the Split Products.- Exercise No. 36 The Methods of Analytic and Preparative Paper Chromatography.- A Preliminary Runs.- B Preparative Procedure.- Exercise No. 37 Separation and Isolation of Peptides or Oligosaccharides by High-Voltage Paper Electrophoresis.- Exercise No. 38 Demonstration of Ion Exchange Column Chromatography of Amino Acids.- Exercise No. 39 Gas-Liquid Chromatography for Fatty Acid Separation,.- Exercise No. 40 Carbohydrate Analysis by Gas-Liquid Chromatography.- Exercise No. 41 Gas-Liquid Chromatographic Analysis of Amino Acids.- Exercise No. 42 Demonstration of Thin-Layer Chromatography.- 2.1.3 Some Other Qualitative Methods for Structural Studies.- Exercise No. 43 Qualitative Analyses of the Constituents of an Unknown Natural Product.- Exercise No. 44 Identification of N-Terminal and C-Terminal Amino Acids.- A N-Terminus.- B C-Terminus 1

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