Beschreibung
Because tumor necrosis factor-a (TNF-a) plays a pivotal role in the regulation of homeostasis and inflammatory immune responses, it offers valuable research opportunities to develop new drugs for the treatment of a wide range of disorders, including cancer, septic shock, rheumatoid arthritis, and other inflammatory diseases. In Tumor Necrosis Factor: Methods and Protocols, well-versed experimentalists survey the basic and translational research being conducted in this field and describe in detail the methods they have developed for TNF production, characterization, mutagenesis, and detection in biological specimens. They also provide several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The protocols presented follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Tumor Necrosis Factor: Methods and Protocols offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of productive, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.
Inhaltsverzeichnis
Tumor Necrosis Factor as a Pharmacological Target Pietro Ghezzi and Anthony Cerami Production and Characterization of Recombinant Human and Murine TNF Flavio Curnis and Angelo Corti Purification of TNF Binding Proteins Hartmut Engelmann, Dani Aderka, and David Wallach Production and Characterization of Receptor-Specific TNF Muteins Paul Ameloot and Peter Brouckaert TNFa Promoter Polymorphysms Silvia Fargion, Luca Valenti, Paola Dongiovanni, and Anna Ludovica Fracanzani Detection of TNF and TNF Receptor mRNA in Cells and Tissues Roberto Furlan, Giuliana Salani, Alessandra Bergami, and Gianvito Martino Immunohistochemical Localization of TNFa and Its Receptors in the Rodent Central Nervous System Paolo Bigini and Tiziana Mennini TNF Signaling: Key Protocols Marta Muzio and Simona Saccani More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D''Herde, and Peter Vandenabeele Assessment of TNFa-Induced Endothelial Damage Through the Loss of Its Barrier Function Elisabetta Ferrero Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression Eleni Douni, Maria Alexiou, and George Kollias TNFa in Experimental Diseases of the CNS Roberto Furlan, Pia Villa, Giorgio Senaldi, and Gianvito Martino In Vivo Vascular Leakage Assay Maria Elena Ferrero Animal Models of Endotoxic Shock Pia Villa and Pietro Ghezzi Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis Richard O. Williams Myocardial Infarction: Animal Models Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, and Roberto Latini Tumor Vascular Therapy With TNF: Critical Review on Animal Models Timo L. M. ten Hagen and Alexander M. M. Eggermont Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting Angelo Corti Index
