Beschreibung
Peroxisome proliferator-activated receptor (PPAR) and sterol regulatory element binding protein (SREBP) families control the transcription of genes involved in lipid metabolism. I investigated the role of PPAR in regulation of lipid metabolism in white adipose tissue (WAT) in response to various metabolic challenges, measuring the expression of lipogenic (SREBP-1c, SREBP-1a, acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS)) and cholesterogenic (SREBP-2, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGCR), and low density lipoprotein-receptor (LDL-R)) genes and flux through the lipogenic pathway. PPAR-deficiency was associated with reduced epididymal fat mass but increased cholesterol concentration, an effect enhanced by feeding a diet enriched in cholesterol. Adipose tissue lipogenesis was increased in PPAR-deficiency, and was further enhanced by feeding a cholesterol-rich diet. The increased lipogenesis in PPAR-deficiency was accompanied by lower mRNA expression of SREBP-1c and its target genes, ACC and FAS. Consumption of a high-cholesterol diet increased the mRNA expression of these genes in PPAR-deficiency.
Autorenporträt
Khwaja Islam is currently Facilities Manager at the University of Oxford. He earned his D.Phil. in Clinical Medicine at Green College, University of Oxford, in Oxford, UK, in 2005, working with Professor Geoff Gibbons and Professor Keith Frayn. His work was on transcriptional regulation of lipid metabolism in white adipose tissue.
