Beschreibung
Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spectroscopic Methods of Analysis: Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods.
Autorenporträt
Inhaltsangabe1. Fluorescene Lifetime Imaging Comes of Age How to Do It and How to Interpert ItYi-Chun Chen, Brayan Q. Spring, and Robert M. Clegg2. Förster Resonance Energy Transfer and Trapping in Selected Systems Analysis by Monte-Carlo SimulationP. Bojarski, A. Synak, L. Kulak, S. Rangelowa-Jankowska, A. Kubicki, B. Grobelna 3. Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence TechniqueWieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski 4. Spectroscopic Probes of RNA Structure and DynamicsKathleen B Hall5. Fluorescence Methods to Study DNA Translocation and Unwinding Kinetics by Nucleic Acid Motors Christopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman 6. Fluorescence Intensity, Anisotropy, and Transient Dynamic Quenching Stopped-Flow KineticsWlodzimierz Bujalowski and Maria J. Jezewska7. Using Structure - Function Constraints in FRET Studies of Large Macromolecular Complexes.§Wlodzimierz Bujalowski and Maria J. Jezewska 8. Illuminating Allostery in Metal Sensing Transcriptional RegulatorsNicholas E. Grossoehme and David P. Giedroc 9. Fluorescence-based BiosensorsMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia, and Sabato D'Auria10. Metal Enhanced ImmunoassaysIgnacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko, Pabak Sarkar, Julian Borejdo, Irina Akopova, Zygmunt Gryczynski 11. Initial Stages of Angiosperm Greening Monitored by Low-Temperature Fluorescence Spectra and Fluorescence LifetimesBeata Mysliwa-Kurdziel, Anna Stecka, Kazimierz Strzalka12. Activation of the Mammalian Cells by using Light-sensitive Ion ChannelsMandy Siu Yu Lung, Paul Pilowsky, Ewa Goldys 13. Detection of Specific Strains of Viable Bacterial Pathogens by using RNA Bead Assays and Flow Cytometry with 2100 BioanalyzerPhilip Butterworth, Henrique T. Baltar M. C. M., Martin Kratzmeier, Ewa M. Goldys 14. Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence TechniquesWieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski, Zygmunt Gryczynski, Ignacy Gryczynski15. Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule Fluorescence SpectroscopyGoran Pljevaljcic, Rae Robertson-Anderson, Edwin van der Schans and David Millar 16. Single-molecule Force Spectroscopy of Polycystic Kidney Disease ProteinsLiang Ma, Meixiang Xu and Andres F. Oberhauser17. Single Molecule Detection Approach to Muscle Study: Kinetics of a Single Cross-Bridge During Contraction of MuscleJulian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla, Rafal Luchowski, Zygmunt Gryczynski, Ignacy Gryczynski 18. Single-Molecule Optical-Trapping Measurements with DNA Anchored to an Array of Gold NanopostsD. Hern Paik & Thomas T. Perkins 19. Electro-optical Analysis of Macromolecular Structure and Dynamics Dietmar Porschke20. Cryoradiolysis and Cryospectroscopy for Studies of Heme-oxygen Intermediates in Cytochromes P450I.G.Denisov, Y.V.Grinkova, S.G.Sliga
