Produktbild: Pediatric Hematology

Pediatric Hematology

Lieferzeit: Lieferbar innerhalb 14 Tagen

106,99 €

inkl. 7 % MwSt. zzgl. Versandkosten

Methods and Protocols, Methods in Molecular Medicine 91

ISBN: 1489939245
ISBN 13: 9781489939241
Herausgeber: Nicholas J Goulden/Colin G Steward
Verlag: Humana Press
Umfang: xii, 272 S.
Erscheinungsdatum: 09.08.2013
Auflage: 1/2013
Produktform: Kartoniert
Einband: Kartoniert

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a goldstandard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

Artikelnummer: 7053629 Kategorie:

Beschreibung

Herstellerkennzeichnung:


Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE

E-Mail: juergen.hartmann@springer.com

Das könnte Ihnen auch gefallen …