Non-Viral Gene Delivery Vectors

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106,99 

Methods and Protocols, Methods in Molecular Biology 1445

ISBN: 1493937162
ISBN 13: 9781493937165
Herausgeber: Gabriele Candiani
Verlag: Humana Press
Umfang: xiv, 290 S., 30 s/w Illustr., 36 farbige Illustr., 290 p. 66 illus., 36 illus. in color.
Erscheinungsdatum: 20.07.2016
Auflage: 1/2016
Produktform: Gebunden/Hardback
Einband: Gebunden

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of nonviral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuliresponsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substratemediated gene deliveryalso termed reverse transfectionand the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and practical, NonViral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

Artikelnummer: 9103646 Kategorie:

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