Beschreibung
Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.
Autorenporträt
InhaltsangabePart I: Infection Models to Study Bacterial Virulence1. Galleria mellonella as an Infection Model for Select Agents Nicolas Sprynski, Eric Valade, and Fabienne Neulat-Ripoll 2. Drosophila as a Model for Intestinal InfectionsMatthieu Lestradet, Kwang-Zin Lee, and Dominique Ferrandon 3. Zebrafish Embryos as a Model to Study Bacterial VirulenceJennifer Mesureur and Annette C. Vergunst 4. Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Bio photonic ImagingGary Splitter, Jerome Harms, Erik Petersen, Diogo Magnani, Marina Durward, Gireesh Rajashekara, and Girish Radhakrishnan 5. Intravital Two photon Imaging to Understand Bacterial Infections of the Mammalian HostFerdinand X. Choong and Agneta Richter-Dahlfors PartII: Cellular Aspects of Host-Bacteria Interactions6. Cre Reporter Assay for Translocation (CRAfT), A Tool for the Study of Protein Translocation into Host CellsAmke den Dulk-Ras, Annette Vergunst, and Paul J.J. Hooykaas 7. Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor TuMonique Barel and Alain Charbit 8. Hijacking the Host Proteasome for the Temporal Degradation of Bacterial EffectorsTomoko Kubori, Andree M. Hubber, and Hiroki Nagai 9. Life Cell Imaging of Phosphoinositide Dynamics during Legionella InfectionStephen Weber and Hubert Hilbi 10. Investigating Interference with Apoptosis Induction by Bacterial ProteinsHua Niu and Yasuko Rikihisa 11. Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial PathogensLan-Ying Lee and Stanton B. Gelvin 12. Investigating TLR Signalling Responses in Murine Dendritic Cells upon Bacterial InfectionSuzana Pinto Salcedo and Lena Alexopoulou Part III: OMICS and Large Scale Screening13. siRNA Screens using Drosophila Cells to Identify Host Factors Required for InfectionAseem Pandey, Sheng Li Ding, Thomas A. Ficht, and Paul de Figueiredo 14. Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host CellsEsteban Chaves-Olarte, Pamela Altamirano-Silva, Caterina Guzmán-Verri, and Edgardo Moreno 15. RNA Sequencing of FACS-sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular PathogensJulien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J. Jansen, Herman P. Spaink, Annemarie H. Meijer 16. Taking the Shortcut for High-throughput Shotgun Proteomic Analysis of BacteriaErica Marie Hartmann, François Allain, Jean-Charles Gaillard, Olivier Pible, and Jean Armengaud 17. Comparative Genomic Analysis at the PATRIC, a Bioinformatic Resource CenterAlice R. Wattam, Joseph L. Gabbard, Maulik Shukla, and Bruno W. Sobral Part IV: Approaches for Difficult Bacteria18. A Markerless Deletion Method for Genetic Manipulation of Burkholderia cenocepacia and Other Multi Antibiotic Resistant Gram-negative BacteriaDaniel F. Aubert, Mohamad A. Hamad, and Miguel A. Valvano 19. Gene Inactivation in Coxiella burnetiiPaul A. Beare and Robert A. Heinzen 20. A Chemical Mutagenesis Approach to Identify Virulence Determinants in the Obligate Intracellular Pathogen Chlamydia trachomatis Bidong Nguyen and Raphael Valdivia
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Humana Press in Springer Science + Business Media
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14197 Berlin
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E-Mail: juergen.hartmann@springer.com
