Produktbild: Epstein Barr Virus

Epstein Barr Virus

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Methods and Protocols, Methods in Molecular Biology 1532

ISBN: 1493966537
ISBN 13: 9781493966530
Herausgeber: Janos Minarovits/Hans Helmut Niller
Verlag: Humana Press
Umfang: xiv, 269 S., 1 s/w Illustr., 21 farbige Illustr., 269 p. 22 illus., 21 illus. in color.
Erscheinungsdatum: 22.11.2016
Auflage: 1/2017
Produktform: Gebunden/Hardback
Einband: Gebunden

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and comprehensive, Epstein BarrVirus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Artikelnummer: 9701353 Kategorie:

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E-Mail: juergen.hartmann@springer.com

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