DNA Methylation Protocols

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165,84 

Methods in Molecular Biology 1708

ISBN: 1493984993
ISBN 13: 9781493984992
Herausgeber: Jörg Tost
Verlag: Humana Press
Umfang: xviii, 704 S., 35 s/w Illustr., 67 farbige Illustr., 704 p. 102 illus., 67 illus. in color.
Erscheinungsdatum: 30.08.2018
Auflage: 3/2018
Produktform: Kartoniert
Einband: Kartoniert

This third edition volume expands on the previous editions with a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.

Artikelnummer: 6778676 Kategorie:

Beschreibung

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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