Beschreibung
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.
Autorenporträt
InhaltsangabePart I. Modern Quantitative Virological Methods 1. Dengue Virus Growth, Purification and Fluorescent Labeling Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, and Eng Eong Ooi 2. Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique Milly M. Choy and Duane J. Gubler 3. Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique A. Ruklanthi de Alwis and Aravinda M. de Silva 4. Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy Joanne L. Tan and Lok Shee Mei 5. Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion Kaiting Ng and John E. Connolly 6. Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-MediatedNeutralization and Enhancement of Dengue Virus Infection Swati Mukherjee, Ted Pierson and Kimberly A. Dowd 7. Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan and Manoj Krishnan Part II. Reverse Genetic Systems to Study Virus Replication and Evolution 8. Development and Application of Dengue Virus Reverse Genetic Systems Andrew Davidson 9. DENV4 Renilla Luciferase Reporter Replicons Sofia Alcaraz-Estrada, Rosa Del Angel, and R. Padmanabhan 10. Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivoRecombination Mark Manzano and R. Padmanabhan 11. Identification of Dengue-Specific Human Antibody Fragments Using Phage DisplayMoon Y.F. Tay, Chin Chin Lee, Subhash G. Vasudevan and Nicole J. Moreland 12. Next Generation Whole Genome Sequencing of Dengue VirusPauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions, and Martin Lloyd Hibberd Part III. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions 13. MPGAfold in Dengue Secondary Structure Prediction Wojciech K. Kasprzak and Bruce A. Shapiro 14. Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE Joanna Sztuba-Solinska and Stuart F.J. Le Grice 15. Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 Kyung H. Choi and Marc Morais 16. Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry A.M. Ward, J. Gunaratne, M. A. Garcia-Blanco 17. Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore Yau Yin Hoe and Susana Geifman Shochat 18. Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation Nicholas J. Barrows, Sharon F. Jamison, Shelton S. Bradrick, Caroline Le Sommer, So Young Kim, James Pearson, and Mariano A. Garcia-Blanco 19. Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import Johanna E. Fraser, Stephen M. Rawlinson, Chunxiao Wang, David A. Jans, and Kylie M. Wagstaff Part IV. In vitro Enzyme Assays for Antiviral Screening 20. Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening Mark Manzano, Janak Padia, and R. Padmanabhan 21. Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral I
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Humana Press in Springer Science + Business Media
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E-Mail: juergen.hartmann@springer.com
