Beschreibung
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
Autorenporträt
Inhaltsangabe1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular ApplicationsNatalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport 2. Two-Photon Excitation Microscopy And Its Applications In NeuroscienceRicardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau 3. Live Spheroid Formation Recorded with Light Sheet-based Fluorescence MicroscopyFrancesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer 4. Fluorescence Microscopy Based RNA Interference ScreeningManuel Gunkel, Nina Beil, Jürgen Beneke, Jürgen Reymann, and Holger Erfle 5. Fluorescence Resonance Energy Transfer Microscopy (FRET)Kasia M. Kedziora and Kees Jalink 6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging MicroscopyYuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy 7. Analysis Of Biomolecular Dynamics By FRAP And Computer SimulationBart Geverts, Martin E. van Royen, and Adriaan B. Houtsmuller 8. Fluorescence Correlation SpectroscopyMark A. Hink 9. Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface MoleculesSuvrajit Saha, Riya Raghupathy, and Satyajit Mayor 10. Practical Structured-Illumination Microscopy E. Hesper Rego and Lin Shao 11. 4Pi Microscopy of the Nuclear Pore ComplexMartin Kahms, Jana Hüve, and Reiner Peters 12. Application Of STED Microscopy To Cell Biology QuestionsNatalia H. Revelo and Silvio O. Rizzoli 13. Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed ProbesKelsey Temprine, Andrew G. York, and Hari Shroff 14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM)Ulrike Endesfelder and Mike Heilemann 15. Optogenetics: Optical Control Of A Photoactivatable Rac In Living CellsTaofei Yin and Yi I. Wu
Herstellerkennzeichnung:
Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE
E-Mail: juergen.hartmann@springer.com
