Microarrays

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160,49 

Preparation, Microfluidics, Detection Methods, and Biological Applications, Integrated Microanalytical Systems, Integrated Analytical Systems

ISBN: 0387727167
ISBN 13: 9780387727165
Herausgeber: Kilian Dill/Robin Liu/Piotr Grodzinsky
Verlag: Springer Verlag GmbH
Umfang: xvi, 356 S.
Erscheinungsdatum: 29.12.2008
Auflage: 1/2009
Format: 2.5 x 24.2 x 16.5
Gewicht: 704 g
Produktform: Gebunden/Hardback
Einband: GEB

The area of microarrays has evolved from genomics, and has been applied to proteomics and single cell studies as well. The applications of microarrays benefit diverse fields ranging from fundamental systems biology to practical diagnostic tests. This book provides a critical analysis of the applications of microarrays in several key areas of bioanalytical research including DNA studies, peptide arrays, protein arrays, combinatorial chemistry arrays, cell-based arrays, and glycoarrays. Further progress in microarrays is being spurred by the advances in micro- and nano-fabrication technologies, modern molecular biology, and data mining. This book is designed to be a useful source of fundamental and current information for microarray research practitioners and for those who are just entering this field.

Artikelnummer: 1931662 Kategorie:

Beschreibung

InhaltsangabeTypes of arrays currently available.- Optical methods of detection of events on the chip surface.- Electrochemical Detection.- Integrated Microfluidic Devices for Automating DNA Microarray Process.- Self-contained, Fully Integrated Biochip for Sample Preparation, PCR Amplification, and DNA Microarray Detection.- Spot identification and data evaluation.- Statistical data analysis.- Electrochemical output and data analysis.- Uses of DNA Microarrays.- Peptide Arrays: towards Routine Implementation.- Antibody Microarrays for the Detection of Toxins.- Autoantibody Profiling Using Synovial Proteome Microarrays.- Carbohydrate microarrays for the Recognition of Microbes and Host Cells.- Development of the Living Cell Array.- Assembly of Multicellular Cell Microarrays Using Magnetic Nanowires.- Combinatorial Organic Chemistry for Future Drug Development and Screening.- Cystic Fibrosis Carrier Screening Test Performed Using a Microarray Platform Based on Electrochemical Detection of DNA Hybridization.- Frequency Dependent and Surface Characterization of DNA Imoobilization and Hybridization (IMEs).- Integrated Potentiostat Circuit for CMOS Sensor Chips.- Use of other redox enzyme systems.- Detection of single micron-sized magnetic bead and magnetic nanoparticles using spin valve sensors for biological applications.- Magnetic Labeling and Detection of Biomolecules using the Bead ARray Counter (BARC) Biosensors.- High Performance Optical Biodetection Using Encapsulated SERS-Active Nanoparticles.- Multiplex sensors using carbon nanotubes and nanowires.- Bar-coding using platforms for nucleic acid and protein recognition.- Electrochemical nanoparticle based sensors.

Inhaltsverzeichnis

Types of arrays currently available.- Optical methods of detection of events on the chip surface.- Electrochemical Detection.- Integrated Microfluidic Devices for Automating DNA Microarray Process.- Self-contained, Fully Integrated Biochip for Sample Preparation, PCR Amplification, and DNA Microarray Detection.- Spot identification and data evaluation.- Statistical data analysis.- Electrochemical output and data analysis.- Uses of DNA Microarrays.- Peptide Arrays: towards Routine Implementation.- Antibody Microarrays for the Detection of Toxins.- Autoantibody Profiling Using Synovial Proteome Microarrays.- Carbohydrate microarrays for the Recognition of Microbes and Host Cells.- Development of the Living Cell Array.- Assembly of Multicellular Cell Microarrays Using Magnetic Nanowires.- Combinatorial Organic Chemistry for Future Drug Development and Screening.- Cystic Fibrosis Carrier Screening Test Performed Using a Microarray Platform Based on Electrochemical Detection of DNA Hybridization.- Frequency Dependent and Surface Characterization of DNA Imoobilization and Hybridization (IMEs).- Integrated Potentiostat Circuit for CMOS Sensor Chips.- Use of other redox enzyme systems.- Detection of single micron-sized magnetic bead and magnetic nanoparticles using spin valve sensors for biological applications.- Magnetic Labeling and Detection of Biomolecules using the Bead ARray Counter (BARC) Biosensors.- High Performance Optical Biodetection Using Encapsulated SERS-Active Nanoparticles.- Multiplex sensors using carbon nanotubes and nanowires.- Bar-coding using platforms for nucleic acid and protein recognition.- Electrochemical nanoparticle based sensors.

Autorenporträt

InhaltsangabeOverview and New Detection Method.- The Current Status of DNA Microarrays.- Electrochemical Detection on Microarrays.- Fluidic Manipulation and Microarrays.- Fully Integrated Microfluidic Device for Direct Sample-to-Answer Genetic Analysis.- Integrated Microfluidic Devices for Automated Microarray-Based Gene Expression and Genotyping Analysis.- Statistical Data Evaluation on Microarrays.- Intensity Concentration Relationships for Electrochemical Detection.- Applications.- Genotyping Arrays.- Peptide-Based Microarray.- Protein Microarrays for the Detection of Biothreats.- Photo-Generation of Carbohydrate Microarrays.- Expression Profiling Using Microfluidic Living Cell Arrays.- New Approaches to the Synthesis of Addressable Microarray Molecular Libraries.- eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening.- Future Improvements in Microarray Sensing.- Use of Redox Enzymes for the Electrochemical Detection of Sequence-Specific DNA and Immunochemical Entities.- Biochip Platforms for DNA Diagnostics.- MagArray Biochips for Protein and DNA Detection with Magnetic Nanotags: Design, Experiment, and Signal-to-Noise Ratio.- Bar Coding Platforms for Nucleic Acid and Protein Detection.- Electrochemical Nanoparticle-Based Sensors.

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