Produktbild: The Bacterial Flagellum

The Bacterial Flagellum

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Methods and Protocols, Methods in Molecular Biology 1593

ISBN: 1493969269
ISBN 13: 9781493969265
Herausgeber: Tohru Minamino/Keiichi Namba
Verlag: Humana Press
Umfang: xi, 326 S., 35 s/w Illustr., 44 farbige Illustr., 326 p. 79 illus., 44 illus. in color. With online files/update.
Erscheinungsdatum: 08.04.2017
Auflage: 1/2017
Produktform: Gebunden/Hardback
Einband: Gebunden

This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.

Artikelnummer: 799500 Kategorie:

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