Mouse Molecular Embryology 1

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213,99 

Methods in Molecular Biology 1092

ISBN: 1603272909
ISBN 13: 9781603272902
Herausgeber: Mark Lewandoski
Verlag: Humana Press
Umfang: xiv, 465 S., 36 s/w Illustr., 71 farbige Illustr., 465 p. 107 illus., 71 illus. in color.
Erscheinungsdatum: 07.12.2013
Auflage: 1/2013
Produktform: Gebunden/Hardback
Einband: GEB

This book details many of the protocols used to study mouse embryology. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

Artikelnummer: 360903 Kategorie:

Beschreibung

Inhaltsangabe1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase StainingYoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina 2. Two Color In Situ Hybridization of Whole-Mount Mouse EmbryosKristin K. Biris and Terry P. Yamaguchi 3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain CryosectionsDavide De Pietri Tonelli, Yoanne M. Clovis and  Wieland B. Huttner  4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray AssaysLatasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd 5. EMAGE: Electronic Mouse Atlas of Gene ExpressionLorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson  6. Real-time PCR Quantification of gene Expression in Embryonic Mouse TissueEric Villalon, David J. Schulz and Samuel T. Waters 7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic ApproachAimin Liu and Jonathan Eggenschwiler 8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene ExpressionDavid A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam 9. Generation of Tissue Organoids by Compaction ReaggregationJulie M. Sheridan and C. Clare Blackburn 10. Ultra-Rapid Vitrification of Mouse Oocytes and EmbryosMark G. Larman PhD and David K. Gardner  11. Mammalian Preimplantation Embryo CultureDavid K. Gardner and Michelle Lane 12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived OrgansJulie Gordon, Billie A. Moore, C. Clare Blackburn and Nancy R. Manley 13. Genetically Encoded Probes Provide a Window On Embryonic ArrhythmiaYvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff 14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model OrganismsSuresh I. Prajapati, Lisa Nevell  and Charles Keller 15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse EmbryosDaisuke Sakai and Paul A Trainor 16. Segmentation and Quantitative Analysis of Individual Cells in Developmental TissuesKaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett 17.     Protein/Peptide Transduction in Metanephric Explant CultureSergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni 18. Direction of Cells Programmed to Die in Mouse EmbroysRocío Hernández-Martínez and Luis Covarrubias 19. Microscopic Computed Tomography-based  Virtual Histology of Embryos Suresh I. Prajapati, David R. Rodriguez, Charles Keller 20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome StudyYelena Golubeva and David Symer 21. The Latest Improvements in the Mouse Sperm PreservationTakehito Kaneko 22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitroSatomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam 23. Using the Textpresso Site Specific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed AllelesBrian G. Condie and William M. Urbanski 24.  Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing

Autorenporträt

Inhaltsangabe1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase StainingYoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina 2. Two Color In Situ Hybridization of Whole-Mount Mouse EmbryosKristin K. Biris and Terry P. Yamaguchi 3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain CryosectionsDavide De Pietri Tonelli, Yoanne M. Clovis and  Wieland B. Huttner  4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray AssaysLatasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd 5. EMAGE: Electronic Mouse Atlas of Gene ExpressionLorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson  6. Real-time PCR Quantification of gene Expression in Embryonic Mouse TissueEric Villalon, David J. Schulz and Samuel T. Waters 7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic ApproachAimin Liu and Jonathan Eggenschwiler 8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene ExpressionDavid A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam 9. Generation of Tissue Organoids by Compaction ReaggregationJulie M. Sheridan and C. Clare Blackburn 10. Ultra-Rapid Vitrification of Mouse Oocytes and EmbryosMark G. Larman PhD and David K. Gardner  11. Mammalian Preimplantation Embryo CultureDavid K. Gardner and Michelle Lane 12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived OrgansJulie Gordon, Billie A. Moore, C. Clare Blackburn and Nancy R. Manley 13. Genetically Encoded Probes Provide a Window On Embryonic ArrhythmiaYvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff 14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model OrganismsSuresh I. Prajapati, Lisa Nevell  and Charles Keller 15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse EmbryosDaisuke Sakai and Paul A Trainor 16. Segmentation and Quantitative Analysis of Individual Cells in Developmental TissuesKaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett 17.     Protein/Peptide Transduction in Metanephric Explant CultureSergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni 18. Direction of Cells Programmed to Die in Mouse EmbroysRocío Hernández-Martínez and Luis Covarrubias 19. Microscopic Computed Tomography-based  Virtual Histology of Embryos Suresh I. Prajapati, David R. Rodriguez, Charles Keller 20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome StudyYelena Golubeva and David Symer 21. The Latest Improvements in the Mouse Sperm PreservationTakehito Kaneko 22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitroSatomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam 23. Using the Textpresso Site Specific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed AllelesBrian G. Condie and William M. Urbanski 24.  Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing

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