Beschreibung
InhaltsangabePart I. Single Molecule Techniques1. Immobilization of Proteins for Single Molecule Fluorescence Resonance Energy Transfer Measurements of Conformation and DynamicsUcheor B. Choi, Keith R. Weninger, and Mark E. Bowen2. Application of Confocal Single Molecule FRET to Intrinsically Disordered ProteinsBenjamin Schuler, Sonja Müller-Späth, Andrea Sorranno, Daniel Nettles3. Single-Molecule Force Spectroscopy of Chimeric Polyprotein Constructs Containing Intrinsically Disordered DomainsMarco Brucale, Isabella Tessari, Luigi Bubacco, Bruno Samorì4. Visualization of Mobility by Atomic Force MicroscopyToshio Ando and Noriyuki Kodera5. Unequivocal Single-Molecule Force Spectroscopy of Intrinsically Disordered ProteinsJavier Oroz, Rubén Hervás, Alejandro Valbuena & Mariano Carrión-VázquezPart II. Methods to Assess Protein Size and Shape6. Sedimentation Velocity Analytical Ultracentrifugation for Intrinsically Disordered ProteinsAndrés G. Salvay, Guillaume Communie, and Christine Ebel7. Analysis of Intrinsically Disordered Proteins by Small-Angle X-ray ScatteringPau Bernadó and Dmitri I. Svergun 8. Small Angle Neutron Scattering (SANS) for the Structural Study of Intrinsically Disordered Proteins in Solution: a Practical GuideFrank Gabel9. Dynamic and Static Light Scattering of Intrinsically Disordered ProteinsKlaus Gast and Christian Fiedler10. Estimation of Intrinsically Disordered Protein Shape and Time-averaged Apparent Hydration in Native Conditions by a Combination of Hydrodynamic MethodsJohanna C. Karst, Ana Cristina Sotomayor-Pérez, Daniel Ladant and Alexandre Chenal11. Size-Exclusion Chromatography in Structural Analysis of Intrinsically Disordered Proteins Vladimir N. UverskyPart III. Methods to Analyze Conformational Behavior12. Denaturant-induced Conformational Transitions in Intrinsically Disordered ProteinsPaolo Neyroz, Stefano Ciurli, and Vladimir N. Uversky13. Identification of Intrinsically Disordered Proteins by a Special 2D ElectrophoresisAgnes Tantos, Peter Tompa14. pH-induced Changes in Intrinsically-disordered ProteinsMatthew D. Smith and Masoud Jelokhani-Niaraki15. Temperature Induced Transitions in Disordered Proteins Probed by NMR SpectroscopyMagnus Kjaergaard, Flemming M. Poulsen and Birthe B. Kragelund16. Analyzing Temperature Induced Transitions in Disordered Proteins by NMR Spectroscopy and Secondary Chemical Shift AnalysesMagnus Kjaergaard, Flemming M. Poulsen & Birthe B. Kragelund17. Osmolyte-, Binding-, and Temperature-Induced Transitions of Intrinsically Disordered ProteinsAllan Chris M. Ferreon and Ashok A. Deniz 18. Laser Temperature-jump Spectroscopy of Intrinsically Disordered ProteinsStephen J. Hagen19. Differential Scanning Microcalorimetry of Intrinsically Disordered Proteins Sergei E. Permyakov20. Identifying Disordered Regions in Proteins by Limited ProteolysisAngelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore and Erica Frare21. The Effect of Counter Ions on the Conformation of Intrinsically Disordered Proteins Studied by Size-exclusion ChromatographyMagdalena Wojtas, Tomasz M. Kaplon, Piotr Dobryszycki, Andrzej Ozyhar22. Mean Net Charge of Intrinsically Disordered Proteins: Experimental Determination of Protein Valence by Electrophoretic Mobility MeasurementsAna Cristina Sotomayor-Pérez, Johanna C. Karst, Daniel Ladant and Alexandre Chenal23. Protein Characterization by Partitioning in Aqueous Two-Phase SystemsLarissa Mikheeva, Pedro Madeira, and Boris Zaslavsky Part IV. Mass-Spectrometry24. Detection and Characterization of Large-scale Protein Conformational Transitions in Solution using Charge-state Distribution A
