Beschreibung
Inhaltsverzeichnis
Section I. Laboratory Essentials 1. The Stem Cell Laboratory: Design, Equipment, and Oversight Robin L. Wesselschmidt and Philip H. Schwartz 2. Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research Lesley Young, Lyn Healy, and Glyn N. Stacey Section II. Derivation 3. Embryonic Stem Cell Derivation from Human Embryos Paul Lerou 4. Derivation of Human Parthenogenetic Stem Cell Lines Nikolay Turovets, Andrey Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa Agapova, and Elena Revazova 5. Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer Justine D. Miller and Thorsten M. Schlaeger 6. Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz 7. Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, and Andras Nagy Section III. Growth, Maintenance and Expansion 8. Traditional Human Embryonic Stem Cell Culture Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, and Alexander E. Stover 9. Xeno-Free Culture of Human Pluripotent Stem Cells Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta 10. Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging Alexander E. Stover and Philip H. Schwartz 11. GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture 12. Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging Victoria Fox Section IV. Characterization 13. Classical Cytogenetics: Karyotyping Techniques Steven E. Bates 14. FISH Analysis of Human Pluripotent Stem Cells Suzanne E. Peterson, Jerold Chun, and Jeanne Loring 15. Immunocytochemical Analysis of Human Pluripotent Stem Cells Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz 16. Flow Cytometric Analysis of Human Pluripotent Stem Cells Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett 17. The Teratoma Assay: An In vivo Assessment of Pluripotency Robin L. Wesselschmidt 18. Detection of Copy Number Variation using SNP Genotyping Gregory M. Cooper and Heather C. Mefford 19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq Michael J. Hitchler and Judd C. Rice 20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring 21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle 22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells Javier Munoz and Albert J. R. Heck Section V. Genetic Manipulation (Gene Modulation) 23. Lentivirus-mediated Modification of Pluripotent Stem Cells Ruchi Bajpai 24. Nucleofection of Human Embryonic Stem Cells Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan 25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells Sujoy K. Dhar ...
