DNA Sequencing Protocols

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106,99 

Methods in Molecular Biology 167

ISBN: 1489942122
ISBN 13: 9781489942128
Herausgeber: Colin A Graham/Alison J M Hill
Verlag: Humana Press
Umfang: x, 244 S., 33 s/w Illustr., 244 p. 33 illus.
Erscheinungsdatum: 01.09.2013
Auflage: 2/2014
Produktform: Kartoniert
Einband: Kartoniert

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. Stateoftheart and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easytofollow methods that will impact today’s genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

Artikelnummer: 7053626 Kategorie:

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Humana Press in Springer Science + Business Media
Heidelberger Platz 3
14197 Berlin
DE

E-Mail: juergen.hartmann@springer.com

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