Chromatin Protocols

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160,49 

Methods in Molecular Biology 1288

ISBN: 1493924737
ISBN 13: 9781493924738
Herausgeber: Srikumar P Chellappan
Verlag: Humana Press
Umfang: xiv, 492 S., 38 s/w Illustr., 36 farbige Illustr., 492 p. 74 illus., 36 illus. in color.
Erscheinungsdatum: 01.04.2015
Auflage: 3/2015
Produktform: Gebunden/Hardback
Einband: Gebunden

Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin.  Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.

Artikelnummer: 7686977 Kategorie:

Beschreibung

Inhaltsangabe1. Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support            Raphael Sandaltzopoulos and Peter B. Becker 2. Preparation and Analysis of Positioned Mononucleosomes            Olga Kulaeva and Vasily M. Studitsky 3. Chromatin Imaging with Time-Lapse Atomic Force Microscopy            Yuri L. Lyubchenko and Luda S. Shlyakhtenko 4. Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR            Toshitsugu Fujita and Hodaka Fujii 5. Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin            Eisuke Gotoh 6. Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes            Melanie Carless 7. Histone Deacetylase Activity Assay            Lirong Peng, Zhigang Yuan, and Edward Seto 8. In Vitro Histone Demethylase Assays            Kenji Kokura, Lidong Sun, and Jia Fang 9. Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution            Carolina E. Pardo, Nancy H. Nabilsi, Russell P. Darst, and Michael P. Kladde 10. Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips            Melanie Carless 11. Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction            Melanie Carless 12. In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination            Heui-Yun Joo, Qian Dai, Amanda E. Jones, Ling Zhai, and Hengbin Wang 13. Immunostaining Analysis of Tissue Cultured Cells and Tissue Sections Using Phospho-Histone H3 (Serine 10)

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