Beschreibung
It is our utmost pleasure to present a new book on tobacco BY-2 cells, Tobacco BY-2 Cells: From Cellular Dynamics to Omics, as the 58th volume in the book series Biotechnology in Agriculture and Forestry (BAF). It represents an ext- sion of the previous book Tobacco BY-2 Cells,vol. 53ofthe BAF. Moreover, the content is rather different from the latter and includes new topics, gleaned from the First International Symposium on Tobacco BY-2 Cells held at the Plant Science Center of the RIKEN, Yokohama, organized by Nagata, Matsuoka and Inze, in September 2004. To this symposium came more than 200 people from different parts of the world to discuss issues. Although most of the contributors to thepreviousvolumeof Tobacco BY-2 Cells gave talks on their subjects, there were many other speakers who presented new topics and approaches. So we enjoyed the symposium very much. Thus we decided to compile a new volume on tobacco BY-2 cells which includes these new topics. In addition, towards the end of the symposium, our common understanding was that the tobacco BY-2 cell system is still important in plant biology, in particular for studying the dynamic features of plant cells. We hope this volume is useful for plant biologists. Contents of the book are as follows: in Chapters I. 1-I. 6, various aspects of the cell cycle and cellular dynamics using BY-2 cells are described. In Ch- ters II. 1-II.
Inhaltsverzeichnis
Section I Cell Cycle and Cellular Dynamics I.1 Novel Approaches for Cell Cycle Analysis in BY-2 Olga A. Koroleva, Gethin R. Roberts, Matthew L. Tomlinson, and John H. Doonan 1 Introduction 2 A Modified alc-Inducible System for Transgene Expression in BY-2 3 Use of the alc System to Define the Time of Action of Induced Protein 4 An Example of Functional Analysis Using the AlcR-GR Gene Switch 5 Transient Protein Expression 6 Emerging Techniques 7 Protocols 8 Composition of Media References I.2 Dynamics and Structure of the Preprophase Band and the Phragmoplast Danny Geelen and Dirk Inze 1 Introduction 2 The Preprophase Band (PPB) 3 The Phragmoplast 4 Future Perspectives References I.3 Formation of Cortical Microtubules in a Cell-Free System Prepared from Plasma Membrane Ghosts and a Cytosolic Extract of BY-2 Cells Takashi Murata and Mitsuyasu Hasebe 1 Introduction 2 Microtubules Nucleate as Branches on Existing Microtubules in the Cortical Arrays of Plant Cells 3 Analysis of the Molecular Mechanisms of Microtubule Nucleation in a BY-2 Cell-Free System 4 Future Perspectives References I.4 Chromosome Dynamics in Tobacco BY-2 Cultured Cells Sachihiro Matsunaga, Nobuko Ohmido, and Kiichi Fukui 1 Introduction 2 Dynamic Analysis of Condensin Complexes 3 Dynamic Analysis of Heterochromatic Protein 1 4 Dynamic Analysis of Aurora Kinases 5 Conclusion and Perspectives References I.5 Ion Channels Meet Cell Cycle Control Rainer Hedrich and Dirk Becker 1 Introduction 2 Cell Elongation 3 Cell Cycle 4 Discussion References I.6 The NACK-PQR MAP Kinase Cascade Controls Plant Cytokinesis Michiko Sasabe, Yuji Takahashi, Takashi Soyano, Hirokazu Tanaka, Ken Kousetsu, Takamasa Suzuki, and Yasunori Machida 1 Introduction 2 Plant Cytokinesis 3 The MAPK Cascade Involved in Cytokinesis 4 Regulation of the NACK-PQR Pathway in the Cell Cycle Machinery 5 Downstream Factors of the NACK-PQR Pathway 6 Future Perspectives References Section II Physiological and Developmental Aspects II.1 Characterization of a Cell Division Factor from Auxin-Autotrophic 2B-13 Cells Derived from the Tobacco BY-2 Cell Line Takashi Shimizu, Kentaro Eguchi, Ikuo Nishida, Kris Laukens, Erwin Witters, and Toshiyuki Nagata 1 Introduction 2 Characterization of Cell Division-Inducing Activity in the Culture Filtrates of 2B-13 Cells 3 Purification of the CDF 4 Searches for CDFs in the Culture Filtrates of Tobacco BY-2 Cells 5 Concluding Remarks and Future Perspectives 6 Protocols References II.2 The BY-2 Cell Line as a Tool to Study Auxin Transport Jan PetraÅ¡ek and Eva ZažÃmalova 1 Introduction 2 Present State of the Art of Cell-to-Cell Transport of Auxins 3 Auxin Transport Studies in Planta 4 Auxin Transport Studies in Simplified Models 5 Concluding Remarks and Future Prospects References II.3 Tobacco BY-2 Cells as a Model for Differentiation in Heterotrophic Plant Cells Yutaka Miyazawa and Atsushi Sakai 1 Introduction 2 Hormonal Factors Affecting Starch-Storing Cell Differentiation in BY-2 Cells 3 Regulations of Gene Expressions Required for Differentiation and Dedifferentiation of Starch-Storing Cells 4 Conclusions and Perspectives References Section III Intracellular Traffic III.1 Imaging the Early Secretory Pathway in BY-2 Cells David G. Robinson and Christophe Ritzenthaler 1 The Early Secretory Pathway in Plants: A Brief Introduction
